Concentration of GPI-anchored proteins upon ER exit in yeast

Article Article scientifique Article Concentration of GPI-anchored proteins upon ER exit in yeast eng 2009 author Castillon Guillaume Alain 560227 author Watanabe Castillon Reika 560847 author Taylor Marcia 626317 author Schwabe Tatjana 623639 author Riezman Howard 134430 186-200 10.1111/j.1600-0854.2008.00857.x 19054390 1398-9219 http://onlinelibrary.wiley.com/store/10.1111/j.1600-0854.2008.00857.x/asset/j.1600-0854.2008.00857.x.pdf?v=1&t=gzs244v3&s=59a9b468e7e386349d9567660fd7a9e18c0c8e55 Previous biochemical work has revealed two parallel routes of exit from the endoplasmic reticulum (ER) in the yeast Saccharomyces cerevisiae, one seemingly specific for glycosyl-phosphatidylinositol (GPI)-anchored proteins. Using the coat protein II (COPII) mutant sec31-1, we visualized ER exit sites (ERES) and identified three distinct ERES populations in vivo. One contains glycosylated pro-alpha-factor, the second contains the GPI-anchored proteins Cwp2p, Ccw14p and Tos6p and the third is enriched with the hexose transporter, Hxt1p. Concentration of GPI-anchored proteins prior to budding requires anchor remodeling, and Hxt1p incorporation into ERES requires the COPII components Sec12p and Sec16p. Additionally, we have found that GPI-anchored protein ER exit is controlled by the p24 family member Emp24p, whereas ER export of most transmembrane proteins requires the Cornichon homologue Erv14p. Amino Acid Transport Systems/genetics/metabolism Cell Wall/genetics/*metabolism Cytoplasmic Vesicles/metabolism Endoplasmic Reticulum/*metabolism Glucose Transport Proteins, Facilitative Glycosylphosphatidylinositols/*metabolism Membrane Glycoproteins/genetics/*metabolism Membrane Proteins/genetics/metabolism Monosaccharide Transport Proteins/genetics/metabolism Saccharomyces cerevisiae/genetics/*metabolism Saccharomyces cerevisiae Proteins/genetics/*metabolism Temperature Vesicular Transport Proteins/genetics/metabolism Dewey 540 39 Département de biochimie 144 false Traffic 10 2 b6d17d5e-93af-42b1-99d5-a784e7892a08 Article Restricted file1.pdf 1347295 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Concentration of GPI-anchored proteins upon ER exit in yeast	Traffic			2009	660	0

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Taylor, Marcia