Scientific article
Open access

Protection of C. elegans from anoxia by HYL-2 ceramide synthase

Published inScience, vol. 324, no. 5925, p. 381-384
Publication date2009

Oxygen deprivation is rapidly deleterious for most organisms. However, Caenorhabditis elegans has developed the ability to survive anoxia for at least 48 hours. Mutations in the DAF-2/DAF-16 insulin-like signaling pathway promote such survival. We describe a pathway involving the HYL-2 ceramide synthase that acts independently of DAF-2. Loss of the ceramide synthase gene hyl-2 results in increased sensitivity of C. elegans to anoxia. C. elegans has two ceramide synthases, hyl-1 and hyl-2, that participate in ceramide biogenesis and affect its ability to survive anoxic conditions. In contrast to hyl-2(lf) mutants, hyl-1(lf) mutants are more resistant to anoxia than normal animals. HYL-1 and HYL-2 have complementary specificities for fatty acyl chains. These data indicate that specific ceramides produced by HYL-2 confer resistance to anoxia.

  • Animals
  • Apoptosis
  • Caenorhabditis elegans/cytology/genetics/physiology
  • Caenorhabditis elegans Proteins/genetics/metabolism
  • Cell Hypoxia
  • Ceramides/biosynthesis/physiology
  • Gene Deletion
  • Genes, Helminth
  • Mutation
  • Oxidoreductases/genetics/metabolism
  • Oxygen/physiology
  • Receptor, Insulin/genetics/metabolism
  • Saccharomyces cerevisiae/genetics/growth & development/physiology
  • Sphingomyelins/biosynthesis/physiology
  • Substrate Specificity
  • Transformation, Genetic
  • Transgenes
Citation (ISO format)
MENUZ, Vincent Régis et al. Protection of C. elegans from anoxia by HYL-2 ceramide synthase. In: Science, 2009, vol. 324, n° 5925, p. 381–384. doi: 10.1126/science.1168532
Main files (1)
Article (Published version)
ISSN of the journal0036-8075

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