Protection of C. elegans from anoxia by HYL-2 ceramide synthase

Article Article scientifique Article Protection of C. elegans from anoxia by HYL-2 ceramide synthase eng 2009 author Menuz Vincent Régis 508810 author Howell Kate S author Gentina Sébastien 555360 author Epstein Sharon 653155 author Riezman Isabelle 23201 author Fornallaz-Mulhauser Monique 25869 author Hengartner Michael O author Gomez Marie author Riezman Howard 134430 author Martinou Jean-Claude 27293 381-384 10.1126/science.1168532 0036-8075 Oxygen deprivation is rapidly deleterious for most organisms. However, Caenorhabditis elegans has developed the ability to survive anoxia for at least 48 hours. Mutations in the DAF-2/DAF-16 insulin-like signaling pathway promote such survival. We describe a pathway involving the HYL-2 ceramide synthase that acts independently of DAF-2. Loss of the ceramide synthase gene hyl-2 results in increased sensitivity of C. elegans to anoxia. C. elegans has two ceramide synthases, hyl-1 and hyl-2, that participate in ceramide biogenesis and affect its ability to survive anoxic conditions. In contrast to hyl-2(lf) mutants, hyl-1(lf) mutants are more resistant to anoxia than normal animals. HYL-1 and HYL-2 have complementary specificities for fatty acyl chains. These data indicate that specific ceramides produced by HYL-2 confer resistance to anoxia. Animals Apoptosis Caenorhabditis elegans/cytology/genetics/physiology Caenorhabditis elegans Proteins/genetics/metabolism Cell Hypoxia Ceramides/biosynthesis/physiology Gene Deletion Genes, Helminth Mutation Oxidoreductases/genetics/metabolism Oxygen/physiology Receptor, Insulin/genetics/metabolism Saccharomyces cerevisiae/genetics/growth & development/physiology Sphingomyelins/biosynthesis/physiology Substrate Specificity Transformation, Genetic Transgenes Dewey 540 Dewey 570 39 Département de biochimie 144 46 Département de biologie cellulaire 165 false Science 324 5925 6e47a623-3dd2-4d4c-bbea-565169447e12 Article (Published version) Public menuz.pdf 611500 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie cellulaire

Title	Published in	Year	Views	Downloads

Protection of C. elegans from anoxia by HYL-2 ceramide synthase	Science	2009	832	919
Identification of genes involded in the adaptation of "Caenorhabditis elegans" to anoxia		2008	881	685

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Menuz, Vincent Régis