Scientific article
Open access

Calcium storage in multivesicular endo-lysosome

Published inPhysical biology, vol. 20, no. 6
Publication date2023-10-17
First online date2023-10-17

It is now established that endo-lysosomes, also referred to as late endosomes, serve as intracellular calcium store, in addition to the endoplasmic reticulum. While abundant calcium-binding proteins provide the latter compartment with its calcium storage capacity, essentially nothing is known about the mechanism responsible for calcium storage in endo-lysosomes. In this paper, we propose that the structural organization of endo-lysosomal membranes drives the calcium storage capacity of the compartment. Indeed, endo-lysosomes exhibit a characteristic multivesicular ultrastructure, with intralumenal membranes providing a large amount of additional bilayer surface. We used a theoretical approach to investigate the calcium storage capacity of endosomes, using known calcium binding affinities for bilayers and morphological data on endo-lysosome membrane organization. Finally, we tested our predictions experimentally after Sorting Nexin 3 depletion to decrease the intralumenal membrane content. We conclude that the major negatively-charge lipids and proteins of endo-lysosomes serve as calcium-binding molecules in the acidic calcium stores of mammalian cells, while the large surface area of intralumenal membranes provide the necessary storage capacity.

  • Acidic calcium stores
  • Calcium
  • Endosomes
  • Intralumenal
  • Lysobisphosphatidic acid
  • Lysosomes
  • Multivesicular
Citation (ISO format)
SCOTT, Cameron Christopher et al. Calcium storage in multivesicular endo-lysosome. In: Physical biology, 2023, vol. 20, n° 6. doi: 10.1088/1478-3975/acfe6a
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Article (Published version)
ISSN of the journal1478-3967

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