Calcium storage in multivesicular endo-lysosome

Article Article scientifique Article Calcium storage in multivesicular endo-lysosome eng 2023-10-17 2023-10-17 author Scott Cameron Christopher 0000-0001-5026-3413 Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland.,CCS new address is: Genedata AG, Margarethenstrasse 38, 4053 Basel, Switzerland. 640182 author Wasnik Vaibhav Hemant 0000-0002-4430-1353 Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland.,NCCR Chemical Biology, Departments of Biochemistry and Theoretical Physics, University of Geneva, Geneva, Switzerland.,Wasnik's new address: Department of Physical Sciences, Indian Institute of Technology Goa, Ponda 403401, Goa, India. 862208 Vaibhav Wasnik author Nunes-Hasler Paula 0000-0002-4598-685X Department of Cell Physiology and Metabolism, Rue Michel-Servet, 1, University of Geneva, Geneva, Switzerland.,PNH new address is: Department of Pathology and Immunology, Rue Michel-Servet, 1, University of Geneva, Geneva, Switzerland. 707324 Département de pathologie et immunologie (PATIM) author Demaurex Nicolas 0000-0002-9933-6772 Department of Cell Physiology and Metabolism, Rue Michel-Servet, 1, University of Geneva, Geneva, Switzerland. 24642 Département de physiologie cellulaire et métabolisme (PHYME) author Kruse Karsten 0000-0001-8665-2349 Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland.,NCCR Chemical Biology, Departments of Biochemistry and Theoretical Physics, University of Geneva, Geneva, Switzerland.,Department of Theoretical Physics, University of Geneva, 24 Quai Ernest Ansermet, 1211 Geneva, Switzerland. 861969 Département de biochimie (CHIBI) author Gruenberg Jean 0000-0002-0300-4862 Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland.,NCCR Chemical Biology, Departments of Biochemistry and Theoretical Physics, University of Geneva, Geneva, Switzerland. 29661 Département de biochimie (CHIBI) 10.1088/1478-3975/acfe6a 37769681 1478-3967 It is now established that endo-lysosomes, also referred to as late endosomes, serve as intracellular calcium store, in addition to the endoplasmic reticulum. While abundant calcium-binding proteins provide the latter compartment with its calcium storage capacity, essentially nothing is known about the mechanism responsible for calcium storage in endo-lysosomes. In this paper, we propose that the structural organization of endo-lysosomal membranes drives the calcium storage capacity of the compartment. Indeed, endo-lysosomes exhibit a characteristic multivesicular ultrastructure, with intralumenal membranes providing a large amount of additional bilayer surface. We used a theoretical approach to investigate the calcium storage capacity of endosomes, using known calcium binding affinities for bilayers and morphological data on endo-lysosome membrane organization. Finally, we tested our predictions experimentally after Sorting Nexin 3 depletion to decrease the intralumenal membrane content. We conclude that the major negatively-charge lipids and proteins of endo-lysosomes serve as calcium-binding molecules in the acidic calcium stores of mammalian cells, while the large surface area of intralumenal membranes provide the necessary storage capacity. Acidic calcium stores Calcium Endosomes Intralumenal Lysobisphosphatidic acid Lysosomes Multivesicular Dewey 540 Dewey 612 Dewey 500.2 Swiss National Science Foundation Regulation by lipids and proteins of endosome biogenesis: ways into and out of the endosome 159479 CH Projects 134 Département de physiologie cellulaire et métabolisme 21C 39 Département de biochimie 144 37 Département de physique théorique 133 false 168 Signaux intracellulaires 210 1293 Mécanismes de la présentation croisée des antigènes 1011 Physical biology 20 6 eb55f952-811f-4021-a74c-0956c1995f30 Article (Published version) Public Scott_2023_Phys._Biol._20_0660042.pdf 945072 application/pdf CC BY-4.0 0fa7d51f2e0e5335fc4dda5145fcaada91578cca634fee8a229a032debf9d42f openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de physique / Département de physique théorique

Title	Published in	Year	Views	Downloads

Calcium storage in multivesicular endo-lysosome	Physical biology	2023	156	150
Positional Information Readout in Ca²⁺ Signaling	Physical Review Letters	2019	283	250
Accuracy of position determination in Ca²⁺ signaling	Physical Review. E	2019	300	221

Archive ouverte UNIGE

Wasnik, Vaibhav