Développement d'un protocole d'expression, purification et vérification de l'activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum

ContributorsRhouni, Soumaya
Master program titleMaîtrise Universitaire en Pharmacie
Defense date2011

Calcium dependent protein kinases (CDPKs) belong to a well defined gene product family that has only been found in plants and protists. In P. falciparum, five genes have been identified that encode five CDPKs (PfCDPK1-5) with 39% to 56% amino acid sequence identity. Since CDPKs do not exist in vertebrates, they represent promising targets for antimalarial therapy. It is the aim of this project to establish a system that will allow the recombinant expression of soluble and active PfCDPKs using a prokaryotic expression system. The isolated proteins will be purified and analyzed regarding yield, purity, structure, activity, kinetics and inhibition.

  • P. falciparum
  • Expression
  • Purification
  • CDPK
  • Protein kinase
  • Inhibition
Citation (ISO format)
RHOUNI, Soumaya. Développement d’un protocole d’expression, purification et vérification de l’activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum. 2011.
Main files (1)
Master thesis
  • PID : unige:16433

Technical informations

Creation06/10/2011 10:56:00 AM
First validation06/10/2011 10:56:00 AM
Update time03/14/2023 4:52:25 PM
Status update03/14/2023 4:52:25 PM
Last indexation01/29/2024 7:11:27 PM
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