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Développement d’un protocole d’expression, purification et vérification de l’activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum

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Denomination Maîtrise Universitaire en Pharmacie
Defense Maîtrise : Univ. Genève, 2011
Abstract Calcium dependent protein kinases (CDPKs) belong to a well defined gene product family that has only been found in plants and protists. In P. falciparum, five genes have been identified that encode five CDPKs (PfCDPK1-5) with 39% to 56% amino acid sequence identity. Since CDPKs do not exist in vertebrates, they represent promising targets for antimalarial therapy. It is the aim of this project to establish a system that will allow the recombinant expression of soluble and active PfCDPKs using a prokaryotic expression system. The isolated proteins will be purified and analyzed regarding yield, purity, structure, activity, kinetics and inhibition.
Keywords P. falciparumExpressionPurificationCDPKProtein kinaseInhibition
Stable URL https://archive-ouverte.unige.ch/unige:16433
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Deposited on : 2011-06-27

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