Développement d'un protocole d'expression, purification et vérification de l'activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum

Diplôme Master Développement d'un protocole d'expression, purification et vérification de l'activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum fre 2011 author Rhouni Soumaya 656260 director Scapozza Leonardo 600318 director Lauciello Leonardo 650644 Calcium dependent protein kinases (CDPKs) belong to a well defined gene product family that has only been found in plants and protists. In P. falciparum, five genes have been identified that encode five CDPKs (PfCDPK1-5) with 39% to 56% amino acid sequence identity. Since CDPKs do not exist in vertebrates, they represent promising targets for antimalarial therapy. It is the aim of this project to establish a system that will allow the recombinant expression of soluble and active PfCDPKs using a prokaryotic expression system. The isolated proteins will be purified and analyzed regarding yield, purity, structure, activity, kinetics and inhibition. P. falciparum Expression Purification CDPK Protein kinase Inhibition Dewey 615 Maîtrise Universitaire en Pharmacie 31 Section des sciences pharmaceutiques 17 false 219d4fde-e1c1-4608-b65b-47853f957121 Master thesis Restricted TPR_Rhouni_Soumaya.pdf 4730719 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Développement d'un protocole d'expression, purification et vérification de l'activité de la Protéine Kinase Dépendante du Calcium 2 du Plasmodium falciparum				2011	357	6

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Rhouni, Soumaya