en
Scientific article
Open access
English

Neurothreads: Development of supportive carriers for mature dopaminergic neuron differentiation and implantation

Published inBiomaterials, vol. 270, 120707
Publication date2021-03
First online date2021-02-02
Abstract

In this study we present the use of elastic macroporous cryogels for differentiation and transplantation of mature neurons. We develop a coating suitable for long-term neuronal culture, including stem cell differentiation, by covalent immobilization of neural adhesion proteins. In the context of cell therapy for Parkinson's disease, we show compatibility with established dopaminergic differentiation of both immortalized mesencephalic progenitors - LUHMES - and human embryonic stem cells (hESCs). We adjust structural properties of the biomaterial to create carriers - Neurothreads - favourable for cell viability during transplantation. Finally, we show feasibility of preservation of mature neurons, supported by Neurothreads, one month after in-vivo transplantation. Preliminary data suggests that the Neurothread approach could provide more mature and less proliferative cells in vivo.

eng
Keywords
  • Cryogel scaffold
  • Hydrogel functionalization
  • In-vivo neuronal transplantation
  • Parkinson's disease
  • Stem cell differentiation
  • Stereotactic injection
  • Cell Differentiation
  • Dopaminergic Neurons
  • Humans
  • Mesencephalon
  • Neurogenesis
  • Parkinson Disease / therapy
Citation (ISO format)
FILIPPOVA, Aleksandra et al. Neurothreads: Development of supportive carriers for mature dopaminergic neuron differentiation and implantation. In: Biomaterials, 2021, vol. 270, p. 120707. doi: 10.1016/j.biomaterials.2021.120707
Main files (1)
Article (Published version)
Identifiers
ISSN of the journal0142-9612
128views
69downloads

Technical informations

Creation03/02/2022 2:38:00 PM
First validation03/02/2022 2:38:00 PM
Update time03/16/2023 2:47:39 AM
Status update03/16/2023 2:47:38 AM
Last indexation02/01/2024 7:52:29 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack