Neurothreads: Development of supportive carriers for mature dopaminergic neuron differentiation and implantation

Article Article scientifique Article Neurothreads: Development of supportive carriers for mature dopaminergic neuron differentiation and implantation eng 2021-02-02 2021-03 author Filippova Aleksandra 778616 author Bonini Fabien 863519 author Efremova Liudmila 865689 author Locatelli Manon 870687 author Preynat-Seauve Olivier 561776 author Beduer Amélie 862936 author Krause Karl-Heinz 23022 author Braschler Thomas Michaël 848483 120707 10.1016/j.biomaterials.2021.120707 33601130 0142-9612 In this study we present the use of elastic macroporous cryogels for differentiation and transplantation of mature neurons. We develop a coating suitable for long-term neuronal culture, including stem cell differentiation, by covalent immobilization of neural adhesion proteins. In the context of cell therapy for Parkinson's disease, we show compatibility with established dopaminergic differentiation of both immortalized mesencephalic progenitors - LUHMES - and human embryonic stem cells (hESCs). We adjust structural properties of the biomaterial to create carriers - Neurothreads - favourable for cell viability during transplantation. Finally, we show feasibility of preservation of mature neurons, supported by Neurothreads, one month after in-vivo transplantation. Preliminary data suggests that the Neurothread approach could provide more mature and less proliferative cells in vivo. Cryogel scaffold Hydrogel functionalization In-vivo neuronal transplantation Parkinson's disease Stem cell differentiation Stereotactic injection Cell Differentiation Dopaminergic Neurons Humans Mesencephalon Neurogenesis Parkinson Disease / therapy Dewey 616.07 137 Département de pathologie et immunologie 21F false 38 Radicaux libres et cellules souches embryonnaires 60 Biomaterials 270 d5ae87b7-c19a-4bde-9eea-4e7d68e380ce Article (Published version) Public 1-s2.0-S0142961221000582-main.pdf 8599458 application/pdf CC BY-NC-ND-4.0 openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Neurothreads: Development of supportive carriers for mature dopaminergic neuron differentiation and implantation	Biomaterials			2021	242	204

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Efremova, Liudmila