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Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum

Cutando, Laura
Puighermanal, Emma
Tarot, Pauline
Bertaso, Federica
Bonnavion, Patricia
de Kerchove d'Exaerde, Alban
Isingrini, Elsa
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Published in Addiction Biology. 2020, e12995
Abstract Prescription stimulants, such as d-amphetamine or methylphenidate are used to treat suffering from attention-deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) and cause phosphorylation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluA1 in the striatum. Whether other brain regions are also affected remains elusive. Here, we demonstrate that d-amphetamine and methylphenidate increase phosphorylation at Ser845 (pS845-GluA1) in the membrane fraction of mouse cerebellum homogenate. We identify Bergmann glial cells as the source of pS845-GluA1 and demonstrate a requirement for intact NE release. Consequently, d-amphetamine-induced pS845-GluA1 was prevented by β1-adenoreceptor antagonist, whereas the blockade of DA D1 receptor had no effect. Together, these results indicate that NE regulates GluA1 phosphorylation in Bergmann glial cells in response to prescription stimulants.
PMID: 33368923
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Research group Mécanismes cellulaires de la dépendance et de l'addiction (520)
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CUTANDO, Laura et al. Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum. In: Addiction Biology, 2020, p. e12995. doi: 10.1111/adb.12995 https://archive-ouverte.unige.ch/unige:150121

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Deposited on : 2021-03-05

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