Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum

Article Article scientifique Article Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum eng 2020 author Cutando Laura author Puighermanal Emma author Castell Almuni Laia 928479 author Tarot Pauline author Bertaso Federica author Bonnavion Patricia author de Kerchove d'Exaerde Alban author Isingrini Elsa author Galante Micaela author Dallerac Glenn author Pascoli Vincent Jean 703155 author Luscher Christian 34378 author Giros Bruno author Valjent Emmanuel e12995 10.1111/adb.12995 33368923 1355-6215 Prescription stimulants, such as d-amphetamine or methylphenidate are used to treat suffering from attention-deficit hyperactivity disorder (ADHD). They potently release dopamine (DA) and norepinephrine (NE) and cause phosphorylation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunit GluA1 in the striatum. Whether other brain regions are also affected remains elusive. Here, we demonstrate that d-amphetamine and methylphenidate increase phosphorylation at Ser845 (pS845-GluA1) in the membrane fraction of mouse cerebellum homogenate. We identify Bergmann glial cells as the source of pS845-GluA1 and demonstrate a requirement for intact NE release. Consequently, d-amphetamine-induced pS845-GluA1 was prevented by β1-adenoreceptor antagonist, whereas the blockade of DA D1 receptor had no effect. Together, these results indicate that NE regulates GluA1 phosphorylation in Bergmann glial cells in response to prescription stimulants. Dewey 616.8 53 Département de neurosciences fondamentales 21A false 271 Mécanismes cellulaires de la dépendance et de l'addiction 520 Addiction Biology c75a9588-e9b9-4519-a494-c44860c1a757 Article (Published version) Restricted AddictionBiologyLC2020.pdf 4253227 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales

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Regulation of GluA1 phosphorylation by d-amphetamine and methylphenidate in the cerebellum	Addiction Biology			2020	226	0

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Castell Almuni, Laia