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Title

Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes

Authors
Kalandadze, Vakhtang
Follenzi, Antonia
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Published in Nature Communications. 2019, vol. 10, no. 1, p. 4491
Abstract Maintaining long-term euglycemia after intraportal islet transplantation is hampered by the considerable islet loss in the peri-transplant period attributed to inflammation, ischemia and poor angiogenesis. Here, we show that viable and functional islet organoids can be successfully generated from dissociated islet cells (ICs) and human amniotic epithelial cells (hAECs). Incorporation of hAECs into islet organoids markedly enhances engraftment, viability and graft function in a mouse type 1 diabetes model. Our results demonstrate that the integration of hAECs into islet cell organoids has great potential in the development of cell-based therapies for type 1 diabetes. Engineering of functional mini-organs using this strategy will allow the exploration of more favorable implantation sites, and can be expanded to unlimited (stem-cell-derived or xenogeneic) sources of insulin-producing cells.
Keywords Diabetes Mellitus, Type 1 / therapyEpithelial Cells / metabolismIslets of Langerhans Transplantation / methodsOrganoids / transplantationTissue Engineering / methods
Identifiers
PMID: 31582751
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Other version: https://www.nature.com/articles/s41467-019-12472-3
Structures
Research groups La transplantation d'îlots de Langerhans (623)
Création de tissus par bio-ingénieurie et régénération d'organes (1021)
Projects FNS: 310030_173138
Fondation Privée des HUG ("CONFIRM Project"), Fondation Romande de Recherche sur le Diabète, European Foundation for the Study of Diabetes
European Foundation for the Study of Diabetes
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(ISO format)
LEBRETON, Fanny et al. Insulin-producing organoids engineered from islet and amniotic epithelial cells to treat diabetes. In: Nature Communications, 2019, vol. 10, n° 1, p. 4491. doi: 10.1038/s41467-019-12472-3 https://archive-ouverte.unige.ch/unige:134290

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Deposited on : 2020-04-08

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