en
Scientific article
Open access
English

A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection

Published inFrontiers in Microbiology, vol. 8, 2533
Publication date2017
Abstract

We isolated from aerobic and anaerobic blood culture bottles from a febrile patient, a Helicobacter-like Gram negative, rod-shaped bacterium that MALDI-TOF MS failed to identify. Blood agar cultures incubated in a microaerobic atmosphere revealed a motile Gram negative rod, which was oxidase, catalase, nitrate reductase, esterase, and alkaline phosphatase positive. It grew at 42°C with no detectable urease activity. Antimicrobial susceptibility testing showed that the organism was susceptible to beta-lactams, gentamicin, erythromycin, and tetracycline but resistant to ciprofloxacin. Electronic microscopy analysis revealed a 3 × 0.5 μm curved rod bacterium harboring two sheathed amphitrichous flagella. Whole genome sequencing revealed a genome 1,708,265 base-pairs long with a GC content of 37.80% and a total of 1,697 coding sequences. The genomic analyses using the nucleotide sequences of the 16S rRNA gene, hsp60 and gyrB genes, as well as the GyrA protein sequence, and the results of Average Nucleotide Identity and in silico DNA-DNA hybridization suggest evidence for a novel Helicobacter species close to Helicobacter equorum and belonging to the group of enterohepatic Helicobacter species. As soon as the particular peptide mass fingerprint of this pathogen is added to the spectral databases, MALDI-TOF MS technology will improve its identification from clinical specimens, especially in case of "sterile infection". We propose to associate the present strain with the Latin name of the place of isolation; Caesarodunum (Tours, France) and suggest "Helicobacter caesarodunensis" for further description of this new bacterium.

Keywords
  • Helicobacter sp.
  • Electron microscopy
  • Genome content
  • Human infection
  • Phylogeny
Citation (ISO format)
VAN DER MEE-MARQUET, Nathalie L et al. A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection. In: Frontiers in Microbiology, 2017, vol. 8, p. 2533. doi: 10.3389/fmicb.2017.02533
Main files (1)
Article (Published version)
Secondary files (2)
Identifiers
ISSN of the journal1664-302X
265views
107downloads

Technical informations

Creation10/28/2019 4:32:00 PM
First validation10/28/2019 4:32:00 PM
Update time03/15/2023 6:29:47 PM
Status update03/15/2023 6:29:46 PM
Last indexation05/05/2024 4:01:21 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack