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High capacity in G protein-coupled receptor signaling

Keshelava, Amiran
Hersch, Micha
Bergmann, Sven
Published in Nature communications. 2018, vol. 9, no. 1, 876
Abstract G protein-coupled receptors (GPCRs) constitute a large family of receptors that activate intracellular signaling pathways upon detecting specific extracellular ligands. While many aspects of GPCR signaling have been uncovered through decades of studies, some fundamental properties, like its channel capacity-a measure of how much information a given transmission system can reliably transduce-are still debated. Previous studies concluded that GPCRs in individual cells could transmit around one bit of information about the concentration of the ligands, allowing only for a reliable on or off response. Using muscarinic receptor-induced calcium response measured in individual cells upon repeated stimulation, we show that GPCR signaling systems possess a significantly higher capacity. We estimate the channel capacity of this system to be above two, implying that at least four concentration levels of the agonist can be distinguished reliably. These findings shed light on the basic principles of GPCR signaling.
Keywords Acetylcholine/pharmacologyCalcium/metabolismCell LineCell Membrane/metabolismHEK293 CellsHumansMuscarinic Agonists/pharmacologyReceptorMuscarinic M3/metabolismReceptorsG-Protein-Coupled/agonists/metabolismSignal Transduction/physiology
PMID: 29491460
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Article (Published version) (1.1 MB) - public document Free access
Research group Voies de signalisation oncogéniques (998)
Swiss National Science Foundation: 31003A_138350
Autre: DFG KA2721/2-1
(ISO format)
KESHELAVA, Amiran et al. High capacity in G protein-coupled receptor signaling. In: Nature Communications, 2018, vol. 9, n° 1, p. 876. doi: 10.1038/s41467-018-02868-y

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Deposited on : 2019-11-08

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