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A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains

Published in Proceedings of the National Academy of Sciences. 2010, vol. 107, no. 26, p. 11954-8
Abstract Staphylococcus aureus is an versatile pathogen that can cause life-threatening infections. Depending on the clinical setting, up to 50% of S. aureus infections are caused by methicillin-resistant strains (MRSA) that in most cases are resistant to many other antibiotics, making treatment difficult. The emergence of community-acquired MRSA drastically changed the picture by increasing the risk of MRSA infections. Horizontal transfer of genes encoding for antibiotic resistance or virulence factors is a major concern of multidrug-resistant S. aureus infections and epidemiology. We identified and characterized a type III-like restriction system present in clinical S. aureus strains that prevents transformation with DNA from other bacterial species. Interestingly, our analysis revealed that some clinical MRSA strains are deficient in this restriction system, and thus are hypersusceptible to the horizontal transfer of DNA from other species, such as Escherichia coli, and could easily acquire a vancomycin-resistance gene from enterococci. Inactivation of this restriction system dramatically increases the transformation efficiency of clinical S. aureus strains, opening the field of molecular genetic manipulation of these strains using DNA of exogenous origin.
Keywords Amino Acid SequenceBase SequenceDNA, Bacterial/geneticsDeoxyribonucleases, Type I Site-Specific/antagonists & inhibitors/genetics/metabolismDeoxyribonucleases, Type III Site-Specific/genetics/metabolismEnterococcus faecalis/enzymology/geneticsEscherichia coli/geneticsGene TargetingGene Transfer, HorizontalGenes, BacterialHumansMethicillin Resistance/geneticsMethicillin-Resistant Staphylococcus aureus/enzymology/genetics/isolation & purificationMolecular Sequence DataPlasmids/geneticsSequence Homology, Amino AcidSpecies SpecificityStaphylococcus aureus/enzymology/genetics/isolation & purification
PMID: 20547849
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Other version: http://www.pnas.org/content/107/26/11954.long
Research groups Analyse génomique et fonctionnelle du staphylocoque doré (604)
Acquisition et expression de facteurs de virulence chez Staphylococcus aureus (86)
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CORVAGLIA, Anna Rita et al. A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains. In: Proceedings of the National Academy of Sciences, 2010, vol. 107, n° 26, p. 11954-8. doi: 10.1073/pnas.1000489107 https://archive-ouverte.unige.ch/unige:12062

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Deposited on : 2010-10-11

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