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Scientific article
English

Imaging mass spectrometry using peptide isoelectric focusing

Published inRCM. Rapid communications in mass spectrometry, vol. 22, no. 17, p. 2667-2676
Publication date2008
Abstract

Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The use of Immobilized pH Gradient-IsoElectric Focusing (IPG-IEF) is also a new trend, as the first dimension of separation, in shotgun proteomics. We report a combination of these two outstanding technologies. This approach is based on the separation of shotgun-produced peptides by IPG-IEF. The peptides are then transferred by capillarity to a capture membrane, which is then scanned by the mass spectrometer to generate MS images. This high-throughput methodology allows a preview of the sample to be obtained in a single day. We report the application of this new pipeline for differential comparison of the membrane proteome of two different strains of Staphylococcus aureus bacteria in a proof-of-principle experiment.

Keywords
  • Isoelectric Focusing
  • Membrane Proteins/chemistry
  • Peptide Mapping
  • Proteomics/methods
  • Species Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation/methods
  • Staphylococcus aureus/chemistry/classification
  • Tandem Mass Spectrometry/instrumentation/methods
Citation (ISO format)
VAEZZADEH, Alireza et al. Imaging mass spectrometry using peptide isoelectric focusing. In: RCM. Rapid communications in mass spectrometry, 2008, vol. 22, n° 17, p. 2667–2676. doi: 10.1002/rcm.3658
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ISSN of the journal0951-4198
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