Imaging mass spectrometry using peptide isoelectric focusing

Article Article scientifique Article Imaging mass spectrometry using peptide isoelectric focusing eng 2008 author Vaezzadeh Alireza 558706 author Simicevic Jovan 645687 author Chauvet Alexis author Francois Patrice 0000-0002-0540-750X 28911 author Zimmermann Catherine 82504 author Lescuyer Pierre 0000-0001-6986-8738 562350 author Deshusses Jacques 24866 author Hochstrasser Denis 26422 2667-2676 10.1002/rcm.3658 18677718 0951-4198 Imaging Mass Spectrometry (IMS) has emerged as a powerful technique in the field of proteomics. The use of Immobilized pH Gradient-IsoElectric Focusing (IPG-IEF) is also a new trend, as the first dimension of separation, in shotgun proteomics. We report a combination of these two outstanding technologies. This approach is based on the separation of shotgun-produced peptides by IPG-IEF. The peptides are then transferred by capillarity to a capture membrane, which is then scanned by the mass spectrometer to generate MS images. This high-throughput methodology allows a preview of the sample to be obtained in a single day. We report the application of this new pipeline for differential comparison of the membrane proteome of two different strains of Staphylococcus aureus bacteria in a proof-of-principle experiment. Isoelectric Focusing Membrane Proteins/chemistry Peptide Mapping Proteomics/methods Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation/methods Staphylococcus aureus/chemistry/classification Tandem Mass Spectrometry/instrumentation/methods Dewey 576 Dewey 616 136 Département de biologie structurale et bioinformatique 21E 56 Département de médecine 221 false 211 Auto-immunité, inflammation et athérosclérose 270 RCM. Rapid communications in mass spectrometry 22 17 c94475e8-a420-41a0-ae61-83730cf19bb4 Article (Accepted version) Restricted Article.pdf 493027 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique Faculté de médecine / Section de médecine clinique / Département de médecine

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Imaging mass spectrometry using peptide isoelectric focusing	RCM. Rapid communications in mass spectrometry			2008	667	1

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Simicevic, Jovan