UNIGE document Scientific Article
previous document  unige:115788  next document
add to browser collection

Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics

Greenfield, Andy
Chaboissier, Marie-Christine
Published in Cell Reports. 2019, vol. 26, no. 12, p. 3272-3283.e3
Abstract Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP+ somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination.
Keywords Single-cell RNA-seqSex determinationOvaryTestisGranulosa cellSertoli cellsSupporting cellsProgenitorsDifferentiationLineage specificationCell fate decisionGonad developmentGene expressionTranscriptomics
PMID: 30893600
Full text
Research group Mécanismes moléculaires et génétiques du développement sexuel (778)
FNS: 31003A_173070
FNS: 51PHI0-141994
(ISO format)
STEVANT, Isabelle et al. Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics. In: Cell Reports, 2019, vol. 26, n° 12, p. 3272-3283.e3. doi: 10.1016/j.celrep.2019.02.069 https://archive-ouverte.unige.ch/unige:115788

278 hits



Deposited on : 2019-04-08

Export document
Format :
Citation style :