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Scientific article
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Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics

Published inCell Reports, vol. 26, no. 12, p. 3272-3283.e3
Publication date2019
Abstract

Sex determination is a unique process that allows the study of multipotent progenitors and their acquisition of sex-specific fates during differentiation of the gonad into a testis or an ovary. Using time series single-cell RNA sequencing (scRNA-seq) on ovarian Nr5a1-GFP+ somatic cells during sex determination, we identified a single population of early progenitors giving rise to both pre-granulosa cells and potential steroidogenic precursor cells. By comparing time series single-cell RNA sequencing of XX and XY somatic cells, we provide evidence that gonadal supporting cells are specified from these early progenitors by a non-sex-specific transcriptomic program before pre-granulosa and Sertoli cells acquire their sex-specific identity. In XX and XY steroidogenic precursors, similar transcriptomic profiles underlie the acquisition of cell fate but with XX cells exhibiting a relative delay. Our data provide an important resource, at single-cell resolution, for further interrogation of the molecular and cellular basis of mammalian sex determination.

Keywords
  • Single-cell RNA-seq
  • Sex determination
  • Ovary
  • Testis
  • Granulosa cell
  • Sertoli cells
  • Supporting cells
  • Progenitors
  • Differentiation
  • Lineage specification
  • Cell fate decision
  • Gonad development
  • Gene expression
  • Transcriptomics
Citation (ISO format)
STEVANT, Isabelle et al. Dissecting Cell Lineage Specification and Sex Fate Determination in Gonadal Somatic Cells Using Single-Cell Transcriptomics. In: Cell Reports, 2019, vol. 26, n° 12, p. 3272–3283.e3. doi: 10.1016/j.celrep.2019.02.069
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ISSN of the journal2211-1247
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Creation04/06/2019 3:59:00 PM
First validation04/06/2019 3:59:00 PM
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