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Scientific article
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English

Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures

Published inChemical Science, vol. 10, no. 8, p. 2253-2258
Publication date2019
Abstract

Lipids are essential components of eukaryotic cell membranes and play crucial roles in cellular signaling and metabolism. While increasing evidence shows that the activities of lipids are dependent upon subcellular localization, tools to study local lipid metabolism and signaling are limited. Herein, we report an approach that enabled us to selectively deliver photo-caged lipids into lysosomes and thereafter to quickly release the lipid molecules by illumination. On combining this method with genetic techniques and lipidomics, we were able to investigate the localization-dependent metabolism of an important intermediate of sphingolipid metabolism, sphingosine. Our data reveal a distinct metabolic pattern of lysosomal sphingosine. In general, this method has the potential to serve as a platform to study lysosomal metabolism and signaling of various lipids and metabolites in living cells.

Citation (ISO format)
FENG, Suihan et al. Lysosome-targeted photoactivation reveals local sphingosine metabolism signatures. In: Chemical Science, 2019, vol. 10, n° 8, p. 2253–2258. doi: 10.1039/C8SC03614D
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ISSN of the journal2041-6520
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Creation03/27/2019 10:11:00 AM
First validation03/27/2019 10:11:00 AM
Update time03/15/2023 4:03:02 PM
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