Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

Lei, Ting; Jacob, Sandrine; Ajil-Zaraa, Imen; Dubuisson, Jean-Bernard; Irion, Olivier; Jaconi, Marisa; Feki, Anis

doi:10.1038/cr.2007.61

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Title		Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges
Authors		Lei, Ting Jacob, Sandrine Ajil-Zaraa, Imen Dubuisson, Jean-Bernard Irion, Olivier Jaconi, Marisa Feki, Anis
Published in		Cell Research. 2007, vol. 17, no. 8, p. 682-688
Abstract		Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.
Keywords		Cell Culture Techniques/methods/trends — Cell Proliferation — Culture Media/chemistry/pharmacology — Embryonic Stem Cells/ cytology/drug effects — Humans — Models, Biological
Identifiers		DOI: 10.1038/cr.2007.61 PMID: 17667917
Full text		Article - Limited access to UNIGE Other version: http://www.nature.com/cr/journal/v17/n8/pdf/cr200761a.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Citation (ISO format)		LEI, Ting et al. Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges. In: Cell Research, 2007, vol. 17, n° 8, p. 682-688. https://archive-ouverte.unige.ch/unige:11416