Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

Article Article scientifique Article Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges eng 2007 author Lei Ting 647925 author Jacob Sandrine author Ajil-Zaraa Imen author Dubuisson Jean-Bernard 135306 author Irion Olivier 125635 author Jaconi Marisa author Feki Anis 134105 682-688 10.1038/cr.2007.61 17667917 1001-0602 http://www.nature.com/cr/journal/v17/n8/pdf/cr200761a.pdf Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications. Cell Culture Techniques/methods/trends Cell Proliferation Culture Media/chemistry/pharmacology Embryonic Stem Cells/ cytology/drug effects Humans Models, Biological Dewey 616.07 137 Département de pathologie et immunologie 21F false Cell Research 17 8 fb5cdb1d-89f2-4b11-91d2-eca29aca26c3 Article Restricted file1.pdf 478184 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges	Cell Research			2007	649	0

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