UNIGE document Scientific Article
previous document  unige:11178  next document
add to browser collection
Title

Chemokine-induced cell death in CCR5-expressing neuroblastoma cells

Authors
Cartier, Laetitia
Irminger-Finger, Irmgard
Published in Journal of Neuroimmunology. 2003, vol. 145, no. 1-2, p. 27-39
Abstract CCR5 is expressed in neurons but its function in this cellular context is hitherto poorly understood. We have generated CCR5-expressing SH-SY5Y neuroblastoma cells. CCR5 ligands induced cell death in these cells, but not in control neuroblastoma cells or in CCR5-expressing fibroblasts. CCR5-dependent killing of neuroblastoma cells occurred through apoptosis, since it was accompanied by caspase-3 activation and could be prevented by a caspase-3 inhibitor. Finally, cell killing by activated microglia was more rapid and extensive in CCR5-expressing neuroblastoma cells than in control cells. In summary, CCR5 may act as a death receptor in cells of neuronal lineage and therefore be involved in inflammatory neurodegeneration.
Keywords AnimalsApoptosis/ immunologyCaspase 3Caspases/metabolismCell Culture Techniques/methodsCell Death/immunologyCell Line, TumorChemokines, CC/metabolism/ physiology/ toxicityCoculture TechniquesEnzyme Activation/immunologyGenetic VectorsHumansLigandsMiceMicroglia/immunology/metabolismNIH 3T3 CellsNeuroblastoma/enzymology/ immunology/ pathologyNeurons/enzymology/immunology/pathologyPhagocytosis/immunologyReceptors, CCR5/ biosynthesis/genetics/physiologyTransfection
Identifiers
PMID: 14644028
Full text
Structures
Citation
(ISO format)
CARTIER, Laetitia et al. Chemokine-induced cell death in CCR5-expressing neuroblastoma cells. In: Journal of Neuroimmunology, 2003, vol. 145, n° 1-2, p. 27-39. https://archive-ouverte.unige.ch/unige:11178

180 hits

0 download

Update

Deposited on : 2010-08-27

Export document
Format :
Citation style :