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Scientific article
English

Chemokine-induced cell death in CCR5-expressing neuroblastoma cells

Published inJournal of neuroimmunology, vol. 145, no. 1-2, p. 27-39
Publication date2003
Abstract

CCR5 is expressed in neurons but its function in this cellular context is hitherto poorly understood. We have generated CCR5-expressing SH-SY5Y neuroblastoma cells. CCR5 ligands induced cell death in these cells, but not in control neuroblastoma cells or in CCR5-expressing fibroblasts. CCR5-dependent killing of neuroblastoma cells occurred through apoptosis, since it was accompanied by caspase-3 activation and could be prevented by a caspase-3 inhibitor. Finally, cell killing by activated microglia was more rapid and extensive in CCR5-expressing neuroblastoma cells than in control cells. In summary, CCR5 may act as a death receptor in cells of neuronal lineage and therefore be involved in inflammatory neurodegeneration.

Keywords
  • Animals
  • Apoptosis/ immunology
  • Caspase 3
  • Caspases/metabolism
  • Cell Culture Techniques/methods
  • Cell Death/immunology
  • Cell Line, Tumor
  • Chemokines, CC/metabolism/ physiology/ toxicity
  • Coculture Techniques
  • Enzyme Activation/immunology
  • Genetic Vectors
  • Humans
  • Ligands
  • Mice
  • Microglia/immunology/metabolism
  • NIH 3T3 Cells
  • Neuroblastoma/enzymology/ immunology/ pathology
  • Neurons/enzymology/immunology/pathology
  • Phagocytosis/immunology
  • Receptors, CCR5/ biosynthesis/genetics/physiology
  • Transfection
Citation (ISO format)
CARTIER, Laetitia et al. Chemokine-induced cell death in CCR5-expressing neuroblastoma cells. In: Journal of neuroimmunology, 2003, vol. 145, n° 1-2, p. 27–39.
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ISSN of the journal0165-5728
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