Optimization of 3D-Visualization of Micro-Anatomical Structures of the Human Inner Ear in Osmium Tetroxide Contrast Enhanced Micro-CT Scans

van den Boogert, Thomas; van Hoof, Marc; Handschuh, Stephan; Glueckert, Rudolf; Guinand, Nils; Guyot, Jean-Philippe; Kingma, Herman; Perez Fornos, Angelica; Seppen, Bart; Johnson Chacko, Lejo; Schrott-Fischer, Anneliese; van de Berg, Raymond

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Title		Optimization of 3D-Visualization of Micro-Anatomical Structures of the Human Inner Ear in Osmium Tetroxide Contrast Enhanced Micro-CT Scans
Authors		van den Boogert, Thomas van Hoof, Marc Handschuh, Stephan Glueckert, Rudolf Guinand, Nils Guyot, Jean-Philippe Kingma, Herman Perez Fornos, Angelica Seppen, Bart Johnson Chacko, Lejo Schrott-Fischer, Anneliese van de Berg, Raymond show all authors [1 - 12]
Published in		Frontiers in neuroanatomy. 2018, vol. 12, p. 41
Abstract		Introduction: Knowledge of the neuro-anatomical architecture of the inner ear contributes to the improvement and development of cochlear and vestibular implants. The present knowledge is mainly based on two-dimensional images (histology) or derived models that simplify the complexity of this architecture. This study investigated the feasibility of visualizing relevant neuro-anatomical structures of the inner ear in a dynamic three-dimensional reproduction, using a combination of staining, micro-CT imaging and an image processing algorithm. Methods: Four fresh cadaveric temporal bones were postfixed with osmium tetroxide (OsO4) and decalcified with EDTA. Micro-CT was used for scanning at 10 μm (4 scans) and 5.5 μm (1 scan) voxel resolution. A new image processing algorithm was developed and the scans were visualized in open source software. Results: OsO4 enhanced the contrast in all scans and the visualization was substantially improved by the image processing algorithm. The three-dimensional renderings provided detailed visualization of the whole inner ear. Details were visible up to the size of individual neurons, nerve crossings and the specific neuro-anatomical structures such as the tunnel of Corti. Conclusion: The combination of OsO4, micro-CT and the proposed image processing algorithm provides an accurate and detailed visualization of the three-dimensional micro-anatomy of the human inner ear.
Identifiers		DOI: 10.3389/fnana.2018.00041 PMID: 29872380
Full text		Article (Published version) (3.2 MB) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques
Research group		Neuroprothèses sensorielles pour la restitution de l'audition et l'équilibre (268)
Citation (ISO format)		VAN DEN BOOGERT, Thomas et al. Optimization of 3D-Visualization of Micro-Anatomical Structures of the Human Inner Ear in Osmium Tetroxide Contrast Enhanced Micro-CT Scans. In: Frontiers in neuroanatomy, 2018, vol. 12, p. 41. https://archive-ouverte.unige.ch/unige:109731