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Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro

Barral-Moran, M. J.
Kiss, P. J.
Published in European Journal of Neuroscience. 2005, vol. 21, no. 1, p. 69-80
Abstract Dendritic arbor development of subventricular zone-derived interneurons is a critical step in their integration into functional circuits of the postnatal olfactory bulb. However, the mechanism and molecular control of this process remain unknown. In this study, we have developed a culture model where dendritic development of purified subventricular zone cells proceeds under serum-free conditions in the absence of added growth factors and non-neural cells. We demonstrate that the large majority of these cells in culture express GABA and elaborate dendritic arbors with spine-like protrusions but they do not possess axons. These neurons expressed receptors for neurotrophins including p75, TrkB and TrkC but not TrkA. Application of exogenous neurotrophins, including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and nerve growth factor (NGF), to cultures stimulated dendritic growth and led to more complex dendritic arbors during the initial 3 days in culture. Our results suggest that these effects are independent of Trk receptors and mediated by the p75/ceramide signaling pathway. We also show that brain-derived neurotrophic factor is the only neurotrophin that is able to influence late-phase dendritic development via TrkB receptor activation. These results suggest that dendritic arbor development of subventricular zone-derived cells may be regulated by neurotrophins through the activation of p75 and the TrkB receptor signaling pathways in a sequentially defined temporal pattern.
Keywords Actins/metabolismAnimalsAnimals, NewbornBrain-Derived Neurotrophic Factor/pharmacologyCell DeathCells, CulturedCerebral Ventricles/ cytologyDNA, Complementary/biosynthesisDendrites/drug effects/ physiologyDiagnostic Imaging/methodsGAP-43 Protein/metabolismImmunohistochemistry/methodsIn Situ Nick-End Labeling/methodsIntermediate Filament Proteins/metabolismMicrotubule-Associated Proteins/metabolismNerve Growth Factor/pharmacologyNerve Tissue Proteins/metabolismNeural Cell Adhesion Molecule L1/metabolismNeurons/drug effects/ physiologyNeurotrophin 3/pharmacologyPolymerase Chain Reaction/methodsProto-Oncogene Proteins c-fos/metabolismRatsRats, Sprague-DawleyReceptor, Nerve Growth FactorReceptor, trkA/metabolismReceptor, trkB/ metabolismReceptors, Nerve Growth Factor/ metabolismSialic Acids/metabolismStem Cells/ physiologyTime FactorsTubulin/metabolismGamma-Aminobutyric Acid/metabolism
PMID: 15654844
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Research group Progéniteurs neuronaux
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GASCON, Eduardo et al. Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro. In: European Journal of Neuroscience, 2005, vol. 21, n° 1, p. 69-80. https://archive-ouverte.unige.ch/unige:10286

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Deposited on : 2010-08-06

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