Scientific article

Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro

Published inEuropean journal of neuroscience, vol. 21, no. 1, p. 69-80
Publication date2005

Dendritic arbor development of subventricular zone-derived interneurons is a critical step in their integration into functional circuits of the postnatal olfactory bulb. However, the mechanism and molecular control of this process remain unknown. In this study, we have developed a culture model where dendritic development of purified subventricular zone cells proceeds under serum-free conditions in the absence of added growth factors and non-neural cells. We demonstrate that the large majority of these cells in culture express GABA and elaborate dendritic arbors with spine-like protrusions but they do not possess axons. These neurons expressed receptors for neurotrophins including p75, TrkB and TrkC but not TrkA. Application of exogenous neurotrophins, including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and nerve growth factor (NGF), to cultures stimulated dendritic growth and led to more complex dendritic arbors during the initial 3 days in culture. Our results suggest that these effects are independent of Trk receptors and mediated by the p75/ceramide signaling pathway. We also show that brain-derived neurotrophic factor is the only neurotrophin that is able to influence late-phase dendritic development via TrkB receptor activation. These results suggest that dendritic arbor development of subventricular zone-derived cells may be regulated by neurotrophins through the activation of p75 and the TrkB receptor signaling pathways in a sequentially defined temporal pattern.

  • Actins/metabolism
  • Animals
  • Animals, Newborn
  • Brain-Derived Neurotrophic Factor/pharmacology
  • Cell Death
  • Cells, Cultured
  • Cerebral Ventricles/ cytology
  • DNA, Complementary/biosynthesis
  • Dendrites/drug effects/ physiology
  • Diagnostic Imaging/methods
  • GAP-43 Protein/metabolism
  • Immunohistochemistry/methods
  • In Situ Nick-End Labeling/methods
  • Intermediate Filament Proteins/metabolism
  • Microtubule-Associated Proteins/metabolism
  • Nerve Growth Factor/pharmacology
  • Nerve Tissue Proteins/metabolism
  • Neural Cell Adhesion Molecule L1/metabolism
  • Neurons/drug effects/ physiology
  • Neurotrophin 3/pharmacology
  • Polymerase Chain Reaction/methods
  • Proto-Oncogene Proteins c-fos/metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Nerve Growth Factor
  • Receptor, trkA/metabolism
  • Receptor, trkB/ metabolism
  • Receptors, Nerve Growth Factor/ metabolism
  • Sialic Acids/metabolism
  • Stem Cells/ physiology
  • Time Factors
  • Tubulin/metabolism
  • Gamma-Aminobutyric Acid/metabolism
Citation (ISO format)
GASCON, Eduardo et al. Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitors in vitro. In: European journal of neuroscience, 2005, vol. 21, n° 1, p. 69–80. doi: 10.1111/j.1460-9568.2004.03849.x
Updates (1)
ISSN of the journal0953-816X

Technical informations

Creation08/06/2010 1:46:39 PM
First validation08/06/2010 1:46:39 PM
Update time03/14/2023 3:58:32 PM
Status update03/14/2023 3:58:32 PM
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