Periaqueductal efferents to dopamine and GABA neurons of the VTA

Article Article scientifique Article Periaqueductal efferents to dopamine and GABA neurons of the VTA eng 2018 author Ntamati Rwaka Niels 810981 author Creed Meaghan 796959 author Achargui Ridouane 848799 author Luscher Christian 34378 e0190297 10.1371/journal.pone.0190297 29304042 1932-6203 Neurons in the periaqueductal gray (PAG) modulate threat responses and nociception. Activity in the ventral tegmental area (VTA) on the other hand can cause reinforcement and aversion. While in many situations these behaviors are related, the anatomical substrate of a crosstalk between the PAG and VTA remains poorly understood. Here we describe the anatomical and electrophysiological organization of the VTA-projecting PAG neurons. Using rabies-based, cell type-specific retrograde tracing, we observed that PAG to VTA projection neurons are evenly distributed along the rostro-caudal axis of the PAG, but concentrated in its posterior and ventrolateral segments. Optogenetic projection targeting demonstrated that the PAG-to-VTA pathway is predominantly excitatory and targets similar proportions of Ih-expressing VTA DA and GABA neurons. Taken together, these results set the framework for functional analysis of the interplay between PAG and VTA in the regulation of reward and aversion. initially published as a preprint on bioRxiv: https://doi.org/10.1101/117416 Animals Dopamine/metabolism Female GABAergic Neurons/metabolism Male Mice Ventral Tegmental Area/metabolism/physiology Dewey 616.8 Autre Fondation Privée des Hopitaux Universitaires de Genève European Commission Mesocorticolimbic System: functional anatomy, drug-evoked synaptic plasticity & behavioral correlates of Synaptic Inhibition 322541 MESSI EU FP7 Swiss National Science Foundation Drug-evoked synaptic plasticity of excitatory and inhibitory transmission in mouse models of addiction 149985 CH Projects 53 Département de neurosciences fondamentales 21A 61 Département des neurosciences cliniques 226 false 271 Mécanismes cellulaires de la dépendance et de l'addiction 520 https://goo.gl/wabL6U PloS one 13 1 72703094-b7e0-43ba-9313-3b2a134cbe17 Article (Submitted version) Public 117416.full.pdf 829375 application/pdf CC BY-4.0 58bb44a5-aea7-46fa-b444-6b06002aadee Article (Published version) Public journal.pone.0190297.pdf 4525381 application/pdf CC BY-4.0 4ec08f84-36ac-412b-9f8b-3b0c71a9b467 Erratum Public journal.pone.0219476(1).pdf 170276 application/pdf CC BY-4.0 The following information is missing from the Funding statement: Funding was provided by the European Research Council (ERC Advanced grant MeSSI). 10.1371/journal.pone.0219476 31269079 openAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Periaqueductal efferents to dopamine and GABA neurons of the VTA	PloS one	2018	544	325
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Ntamati Rwaka, Niels