The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Article Article scientifique Article The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A eng 2013 author Kress Elsa 673918 author Schwager Françoise 27468 author Holtackers René author Seiler Jonas author Prodon François 757636 author Zanin Esther author Eiteneuer Annika author Toya Mika author Sugimoto Asako author Meyer Hemmo author Meraldi Patrick 0000-0001-9742-8756 108140 author Gotta Monica 0000-0002-4336-7860 659510 559-575 10.1083/jcb.201209107 23649807 0021-9525 Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved. Adaptor Proteins, Signal Transducing/metabolism/physiology Adenosine Triphosphatases/metabolism Animals Caenorhabditis elegans/cytology/genetics Caenorhabditis elegans Proteins/metabolism/physiology Cell Cycle Cell Cycle Proteins/metabolism Cell Line Centrosome/metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Green Fluorescent Proteins/metabolism Humans Mitosis Protein-Serine-Threonine Kinases/metabolism RNA Interference Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 579 Groupe Meraldi Patrick (physiologie cellulaire et métabolisme) 922 509 La division asymétrique 847 The Journal of cell biology 201 4 c631d2f1-9f8d-45c5-b289-68db2399ad57 Article (Published version) Public J Cell Biol-2013-Kress-559-75(2).pdf 3880121 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

Title	Published in	Year	Views	Downloads

When the Swiss clock goes wrong: Regulation of Aurora A by UBXN-2/CDC-48	Cell cycle	2013	620	2
The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A	The Journal of cell biology	2013	726	591
Aurora A in cell division: kinase activity not required	Nature cell biology	2011	958	826

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