Scientific article
Open access

The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Published inThe Journal of cell biology, vol. 201, no. 4, p. 559-575
Publication date2013

Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

  • Adaptor Proteins, Signal Transducing/metabolism/physiology
  • Adenosine Triphosphatases/metabolism
  • Animals
  • Caenorhabditis elegans/cytology/genetics
  • Caenorhabditis elegans Proteins/metabolism/physiology
  • Cell Cycle
  • Cell Cycle Proteins/metabolism
  • Cell Line
  • Centrosome/metabolism
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Green Fluorescent Proteins/metabolism
  • Humans
  • Mitosis
  • Protein-Serine-Threonine Kinases/metabolism
  • RNA Interference
Citation (ISO format)
KRESS, Elsa et al. The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. In: The Journal of cell biology, 2013, vol. 201, n° 4, p. 559–575. doi: 10.1083/jcb.201209107
Main files (1)
Article (Published version)
ISSN of the journal0021-9525

Technical informations

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