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Title

The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Authors
Holtackers, René
Seiler, Jonas
Zanin, Esther
Eiteneuer, Annika
Toya, Mika
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Published in The Journal of Cell Biology. 2013, vol. 201, no. 4, p. 559-575
Abstract Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.
Keywords Adaptor Proteins, Signal Transducing/metabolism/physiologyAdenosine Triphosphatases/metabolismAnimalsCaenorhabditis elegans/cytology/geneticsCaenorhabditis elegans Proteins/metabolism/physiologyCell CycleCell Cycle Proteins/metabolismCell LineCentrosome/metabolismGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGreen Fluorescent Proteins/metabolismHumansMitosisProtein-Serine-Threonine Kinases/metabolismRNA Interference
Identifiers
PMID: 23649807
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Article (Published version) (3.7 MB) - public document Free access
Structures
Research groups La division asymétrique (847)
Groupe Meraldi Patrick (physiologie cellulaire et métabolisme) (922)
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KRESS, Elsa et al. The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A. In: The Journal of Cell Biology, 2013, vol. 201, n° 4, p. 559-575. https://archive-ouverte.unige.ch/unige:31953

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Deposited on : 2013-12-10

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