The volcano-sedimentary infill of the Dschang basin in the Western part of Cameroon is poorly known. The present study provides the first biostratigraphical data of the volcano-sedimentary sequence and allows constraining the age and the position of this basin within the regional context of Central Africa. The studied sequence is composed of three depositional units. The first unit is a fining-upwards sequence of coarse-grained sandstone that gets finer towards the top, passing to thick layers of fossiliferous claystone. The second unit is strongly affected by volcanic activity and consists of a volcano-sedimentary sequence. The third unit is marked by basaltic volcanism and covers the series. Diverse and well-preserved palynomorphs were recovered and are used to provide valuable information for age determination of the Dschang volcano-sedimentary sequence, namely to be of late Eocene- Early Miocene age. The recovered spores, pollen, green algae and fungal remains from the bottom of the sequence include Bombacidites spp., Clavainaperturites cf. clavatus, Crototricolpites sp., Perfotricolpites digitatus, Proteacidites spp., Psilastephanocolporites minor, Psilastephanocolporites spp., Retitrescolpites spp., Retitricolporites irregularis, Retitricolporites spp., Retitriporites sp., Spirosyncolpites bruni (probably synonymous to Spirosyncolpites spiralis), Striatopollis bellus, (considered synonymous to Striatopollis catatumbus), Striatopollis sp., Tetracolporites quadratus, Tetratricolporites sp., ?Verrutricolporites rotundiporis, Cyathidites sp., Laevigatosporites spp., Polypodiaceoisporites spp., Verrucatosporites usmensis, Verrucatosporites spp., Botryococcus sp., Tasmanites sp., and fungal remains. The samples are assigned to the Verrucatosporites usmensis Zone of Salard-Cheboldaeff (1979). The palynological assemblages are similar to those of other Late Eocene to Early Miocene palynological records from the West, Central and Northern African basins. Study of the organic matter by pyrolysis Rock-Eval 6 (Types I, II and III), the sedimentological setting, the palynofacies and the palynomorph assemblages show evidences of tidal influence (presence of the prasinophyte Tasmanites) in an active continental margin (semi-arid to humid lacustrine depositional environment). The Ngoua sedimentary sequence is fluvio-lacustrine, and believed to have witnessed some occasional marine incursions possibly during the opening of the Central Atlantic Ocean (CAO) and strongly disturbed later by intense volcanic activity.