en
Scientific article
English

ER-mitochondria contacts control surface glycan expression and sensitivity to killer lymphocytes in glioma stem-like cells

Published inEMBO Journal, vol. 36, no. 11, p. 1493-1512
Publication date2017
Abstract

Glioblastoma is a highly heterogeneous aggressive primary brain tumor, with the glioma stem-like cells (GSC) being more sensitive to cytotoxic lymphocyte-mediated killing than glioma differentiated cells (GDC). However, the mechanism behind this higher sensitivity is unclear. Here, we found that the mitochondrial morphology of GSCs modulates the ER-mitochondria contacts that regulate the surface expression of sialylated glycans and their recognition by cytotoxic T lymphocytes and natural killer cells. GSCs displayed diminished ER-mitochondria contacts compared to GDCs. Forced ER-mitochondria contacts in GSCs increased their cell surface expression of sialylated glycans and reduced their susceptibility to cytotoxic lymphocytes. Therefore, mitochondrial morphology and dynamism dictate the ER-mitochondria contacts in order to regulate the surface expression of certain glycans and thus play a role in GSC recognition and elimination by immune effector cells. Targeting the mitochondrial morphology, dynamism, and contacts with the ER could be an innovative strategy to deplete the cancer stem cell compartment to successfully treat glioblastoma.

Keywords
  • Animals
  • Cell Line
  • Endoplasmic Reticulum/metabolism
  • Humans
  • Killer Cells, Natural/immunology
  • Mice
  • Mitochondria/metabolism
  • Neuroglia/physiology
  • Polysaccharides/biosynthesis
  • Stem Cells/physiology
  • T-Lymphocytes, Cytotoxic/immunology
Citation (ISO format)
BASSOY, Esen et al. ER-mitochondria contacts control surface glycan expression and sensitivity to killer lymphocytes in glioma stem-like cells. In: EMBO Journal, 2017, vol. 36, n° 11, p. 1493–1512. doi: 10.15252/embj.201695429
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0261-4189
529views
10downloads

Technical informations

Creation09/26/2017 11:43:00 AM
First validation09/26/2017 11:43:00 AM
Update time03/15/2023 2:03:40 AM
Status update03/15/2023 2:03:39 AM
Last indexation01/17/2024 12:45:48 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack