Dynamic cooperative glycan assembly blocks the binding of bacterial lectins to epithelial cells

Machida, Takuya; Novoa, Alexandre; Gillon, Émilie; Zheng, Shuangshuang; Claudinon, Julie; Eierhoff, Thorsten; Imberty, Anne; Römer, Winfried; Winssinger, Nicolas

doi:10.1002/anie.201700813

Scientific article

English

Dynamic cooperative glycan assembly blocks the binding of bacterial lectins to epithelial cells

Contributors

Machida, Takuya; Novoa, Alexandre; Gillon, Émilie; Zheng, Shuangshuang; Claudinon, Julie; Eierhoff, Thorsten; Imberty, Anne; Römer, Winfried; Winssinger, Nicolas

Published in

Angewandte Chemie, vol. 56, no. 24, p. 6762-6766

Publication date

2017

Abstract

Pathogens frequently rely on lectins for adhesion and cellular entry into the host. Since these interactions typically result from multimeric binding of lectins to cell-surface glycans, novel therapeutic strategies are being developed with the use of glycomimetics as competitors of such interactions. Herein we study the benefit of nucleic acid based oligomeric assemblies with PNA–fucose conjugates. We demonstrate that the interactions of a lectin with epithelial cells can be inhibited with conjugates that do not form stable assemblies in solution but benefit from cooperativity between ligand–protein interactions and PNA hybridization to achieve high affinity. A dynamic dimeric assembly fully blocked the binding of the fucose-binding lectin BambL of Burkholderia ambifaria, a pathogenic bacterium, to epithelial cells with an efficiency of more than 700-fold compared to l-fucose.

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

UNIGE research groups

Groupe Winssinger

Citation (ISO format)

MACHIDA, Takuya et al. Dynamic cooperative glycan assembly blocks the binding of bacterial lectins to epithelial cells. In: Angewandte Chemie, 2017, vol. 56, n° 24, p. 6762–6766. doi: 10.1002/anie.201700813

Article (Published version)

Article (Accepted version)

Identifiers

PID : unige:95405
DOI : 10.1002/anie.201700813

Journal ISSN

1433-7851

600views

299downloads

Creation

11/07/2017 16:31:00

First validation

11/07/2017 16:31:00

Update

13/10/2025 19:55:46

Status update

15/03/2023 01:49:48

Last indexation

01/07/2026 06:11:01

Dynamic cooperative glycan assembly blocks the binding of bacterial lectins to epithelial cells

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (2)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation