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Master
English

In search of epigenetic regulators mediating the long-term effect of Withanolide F in human colon cancer cells

Contributors

Angulo Valdivia, Cecilia

Directors

Ruiz Altaba, Ariel; Bernal Zamora, Carolina; Galliot, Brigitte

Master program title

Master en Biologie, orientation Génétique, développement et évolution (GDE)

Defense date

2017

Abstract

WNT signaling is critical in regulating stem cell behavior and promoting neoplastic transformation in several cancers. A recent study from Ruiz i Altaba's group uncovered a novel WNT inhibitor (Withanolide F) which produces long-term repression of WNT-TCF targets, and is paralleled by changes in the epigenetic landscape in different cancer cells, leading to the possibility that the drug's effect on WNT signaling occurs via epigenetic alterations. We embarked on our analysis in downregulating specific genes which could be potentially involved in its epigenetic modulation and test if the individual gene silencing could recapitulate Withanolide F effects on WNT response. However, our results show that SMARCAL1 knockdown in two primary colon cancer cells do not recapitulate the drug's effects, but generate changes in certain behaviors. Thus, SMARCAL1 knockdown can affect the epigenetic landscape on these cancer cells but does not appear to mimic the effect of Withanolide F treatment.

UNIGE affiliation entities

Citation (ISO format)

ANGULO VALDIVIA, Cecilia. In search of epigenetic regulators mediating the long-term effect of Withanolide F in human colon cancer cells. Master, 2017.

Main files (1)

Master thesis

Identifiers

  • PID : unige:95384
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Technical informations

Creation

05/07/2017 12:49:00

First validation

05/07/2017 12:49:00

Update

15/03/2023 01:49:34

Status update

15/03/2023 01:49:34

Last indexation

31/10/2024 07:18:26
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