Calciosome, a sarcoplasmic reticulum-like organelle involved in intracellular Ca2+-handling by non-muscle cells: studies in human neutrophils and HL-60 cells
|Published in||Cell Calcium. 1989, vol. 10, no. 5, p. 351-361|
|Abstract||Calciosomes are intracellular organelles in HL-60 cells, neutrophils and various other cell types, characterized by their content of a Ca2+-binding protein that is biochemically and immunologically similar to calsequestrin (CS) from muscle cells. In subcellular fractionation studies the CS-like protein copurifies with functional markers of the inositol 1,4,5-trisphosphate (IP3) releasable Ca2+-store. These markers (ATP-dependent Ca2+-uptake and IP3-induced Ca2+-release) show a subcellular distribution which is clearly distinct from the endoplasmic reticulum and other organelles. In morphological studies, antibodies against rabbit skeletal muscle CS protein specifically stained hitherto unrecognized vesicles with a diameter between 50 and 250 nm. Thus both, biochemical and morphological studies indicate that the calsequestrin containing intracellular Ca2+-store, now referred to as the calciosome, is distinct from other known organelles such as endoplasmic reticulum. Calciosomes are likely to play an important role in intracellular Ca2+-homeostasis. They are possibly the intracellular target of inositol 1,4,5-trisphosphate and thus the source of Ca2+ that is redistributed into the cytosol following surface receptor activation in non-muscle cells.|
|Keywords||Biological Transport, Active — Calcium/ metabolism — Calcium Channels/metabolism — Calcium-Binding Proteins/metabolism — Calcium-Transporting ATPases/metabolism — Calsequestrin/metabolism — Cell Line — Endoplasmic Reticulum/metabolism/ultrastructure — Humans — Inositol 1,4,5-Trisphosphate — Inositol Phosphates/physiology — Neutrophils/ metabolism/ultrastructure — Organelles/classification/ metabolism — Sarcoplasmic Reticulum/ metabolism|
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|Research group||Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)|
|KRAUSE, Karl-Heinz et al. Calciosome, a sarcoplasmic reticulum-like organelle involved in intracellular Ca2+-handling by non-muscle cells: studies in human neutrophils and HL-60 cells. In: Cell Calcium, 1989, vol. 10, n° 5, p. 351-361. https://archive-ouverte.unige.ch/unige:9380|