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Title

Calciosome, a sarcoplasmic reticulum-like organelle involved in intracellular Ca2+-handling by non-muscle cells: studies in human neutrophils and HL-60 cells

Authors
Volpe, P.
Pozzan, T.
Meldolesi, J.
Published in Cell Calcium. 1989, vol. 10, no. 5, p. 351-361
Abstract Calciosomes are intracellular organelles in HL-60 cells, neutrophils and various other cell types, characterized by their content of a Ca2+-binding protein that is biochemically and immunologically similar to calsequestrin (CS) from muscle cells. In subcellular fractionation studies the CS-like protein copurifies with functional markers of the inositol 1,4,5-trisphosphate (IP3) releasable Ca2+-store. These markers (ATP-dependent Ca2+-uptake and IP3-induced Ca2+-release) show a subcellular distribution which is clearly distinct from the endoplasmic reticulum and other organelles. In morphological studies, antibodies against rabbit skeletal muscle CS protein specifically stained hitherto unrecognized vesicles with a diameter between 50 and 250 nm. Thus both, biochemical and morphological studies indicate that the calsequestrin containing intracellular Ca2+-store, now referred to as the calciosome, is distinct from other known organelles such as endoplasmic reticulum. Calciosomes are likely to play an important role in intracellular Ca2+-homeostasis. They are possibly the intracellular target of inositol 1,4,5-trisphosphate and thus the source of Ca2+ that is redistributed into the cytosol following surface receptor activation in non-muscle cells.
Keywords Biological Transport, ActiveCalcium/ metabolismCalcium Channels/metabolismCalcium-Binding Proteins/metabolismCalcium-Transporting ATPases/metabolismCalsequestrin/metabolismCell LineEndoplasmic Reticulum/metabolism/ultrastructureHumansInositol 1,4,5-TrisphosphateInositol Phosphates/physiologyNeutrophils/ metabolism/ultrastructureOrganelles/classification/ metabolismSarcoplasmic Reticulum/ metabolism
Identifiers
PMID: 2548728
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Structures
Research group Staphylocoques dorés résistants à la méthicilline et hygiène hospitalière (330)
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KRAUSE, Karl-Heinz et al. Calciosome, a sarcoplasmic reticulum-like organelle involved in intracellular Ca2+-handling by non-muscle cells: studies in human neutrophils and HL-60 cells. In: Cell Calcium, 1989, vol. 10, n° 5, p. 351-361. https://archive-ouverte.unige.ch/unige:9380

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Deposited on : 2010-07-12

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