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Scientific article
English

Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfaces

Contributors

Francois, Patrice; Letourneur, D.; Lew, Daniel Pablo; Jozefonwicz, J.; Vaudaux, Pierre

Published in

Journal of biomaterials science. Polymer ed, vol. 10, no. 12, p. 1207-1221

Publication date

1999

Abstract

The ability of Staphylococcus aureus to recognize several extracellular matrix or plasma proteins (e.g., fibrinogen, fibronectin, and collagen) promotes bacterial attachment to artificial surfaces. Whereas most S. aureus clinical isolates elaborate a wide repertoire of bacterial surface receptors' called adhesins, exhibiting specific binding of individual host proteins, S. epidermidis is lacking most of such protein adhesins. To document the interactions between S. epidermidis and various surface-adsorbed proteins, we first compared promotion of bacterial attachment by seven purified human proteins immobilized onto poly(methyl methacrylate) (PMMA) coverslips. Only two of them, namely fibronectin and fibrinogen, exhibited adhesion-promoting activities. In the presence of native heparin or two functionalized dextrans (CMDBS for Carboxy Methyl, Benzylamide sulfonate/sulfate), a dose-dependent inhibition of S. epidermidis adhesion to fibronectin-coated, but not to fibrinogen-coated surfaces was observed. The inhibitory effects of each CMDBS were much stronger than that of native heparin. In contrast, a control highly negatively charged, dextran exclusively substituted with carboxy methyl groups exerted no inhibition on S. epidermidis adhesion. To evaluate how CMDBS could interfere with S. epidermidis attachment to coverslips coated in vivo with extracellular matrix components, we also tested PMMA surfaces retrieved from tissue cages subcutaneously implanted in guinea pigs. Each CMDBS, but not heparin, strongly inhibited S. epidermidis adhesion to explanted coverslips, even in the presence of tissue cage fluid. In conclusion, fibronectin plays an important role in promoting S. epidermidis attachment to implanted biomaterials. Furthermore, S. epidermidis adhesion to fibronectin-coated or implanted biomaterials can be efficiently blocked in vitro by CMDBS.

Keywords

  • Animals
  • Biocompatible Materials
  • Cell Adhesion/ drug effects
  • Complement Inactivator Proteins/pharmacology
  • Dextrans/ pharmacology
  • Diffusion Chambers, Culture
  • Extracellular Matrix Proteins/metabolism
  • Fibrinogen/metabolism
  • Fibronectins/ metabolism
  • Guinea Pigs
  • Heparin/ pharmacology
  • Humans
  • Polymers/ metabolism
  • Prostheses and Implants
  • Staphylococcus epidermidis/cytology/ metabolism
  • Surface Properties

UNIGE affiliation entities

Citation (ISO format)

FRANCOIS, Patrice et al. Inhibition by heparin and derivatized dextrans of Staphylococcus epidermidis adhesion to in vitro fibronectin-coated or explanted polymer surfaces. In: Journal of biomaterials science. Polymer ed, 1999, vol. 10, n° 12, p. 1207–1221. doi: 10.1163/156856299x00027

Identifiers

Additional URL for this publication

http://www.ingentaconnect.com/content/vsp/bsp/1999/00000010/00000012/art00002?token=00501a94f155cf8c816720297d7634257b742b467a3f7b2d38496d3f6a4b6e4e395e4e6b63313120

Journal ISSN

0920-5063
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Technical informations

Creation

12/07/2010 14:36:48

First validation

12/07/2010 14:36:48

Update

14/03/2023 15:54:19

Status update

14/03/2023 15:54:19

Last indexation

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