UNIGE document Scientific Article
previous document  unige:9252  next document
add to browser collection
Title

Differences in aggregation properties and levels of the neural cell adhesion molecule (NCAM) between islet cell types

Authors
Rouiller, D. G.
Cirulli, V.
Published in Experimental Cell Research. 1990, vol. 191, no. 2, p. 305-312
Abstract Cells within rat islets of Langerhans are typically organized as a core of B-cells, surrounded by the other cell types. When mixed in culture, primary islet cells and insulinoma (RIN2A) cells form aggregates where B-cells are centrally located, surrounded by non-B-cells, while RIN-cells segregate as the outermost layer. To gain insight into the molecular basis underlying this nonrandom cellular organization, the aggregation properties of the three cell populations were studied. Isolated islet cells were separated into B-cells and non-B-cells by autofluorescence-activated cell sorting (FACS). In a short-term aggregation assay, primary B-cell aggregation in the absence of calcium was only 19 +/- 3.7%, compared to the 67 +/- 2.9% seen in the presence of calcium (mean +/- SEM; P less than 0.001; n = 7). By contrast, non-B-cell aggregation and RIN cell aggregation in the absence of calcium (62 +/- 2 and 66 +/- 2%, respectively) were only slightly less than with calcium (70 +/- 3 and 76 +/- 3%). The surface density of the Ca2(+)-independent neural CAM (NCAM) was therefore measured by flow cytometry and found to be 2.64 +/- 0.82-fold higher in non-B-cells, compared to that in B-cells (P less than 0.01; n = 3). Even higher levels were found on RIN cells. In the three cell types, NCAM-140 was the only molecular form detected by immunoblotting. In conclusion, differences in the calcium dependency of aggregation and in the levels of NCAM are demonstrated among islet B-cells, non-B-cells, and RIN cells. Because cell-cell adhesion is crucial for the maintenance of adult tissue, these aggregation specificities might contribute to the concentric segregation of islet cell types in culture and to the nonrandom distribution of cells within rat islets.
Keywords AnimalsCalcium/pharmacologyCell Adhesion Molecules, Neuronal/analysis/genetics/ metabolismCell Aggregation/drug effects/physiologyCell LineCell SeparationFlow CytometryGene Expression/drug effectsImmunoblottingInsulinoma/chemistry/metabolism/pathologyIslets of Langerhans/chemistry/ cytology/metabolismPancreatic Neoplasms/chemistry/metabolism/pathologyRatsRats, Inbred Strains
Identifiers
PMID: 2257882
Full text
This document has no fulltext available yet, but you can contact its author by using the form below.
Structures
Citation
(ISO format)
ROUILLER, D. G., CIRULLI, V., HALBAN, Philippe A. Differences in aggregation properties and levels of the neural cell adhesion molecule (NCAM) between islet cell types. In: Experimental Cell Research, 1990, vol. 191, n° 2, p. 305-312. https://archive-ouverte.unige.ch/unige:9252

209 hits

0 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :