UNIGE document Scientific Article
previous document  unige:91539  next document
add to browser collection
Title

Critical Analysis of Rate Constants and Turnover Frequency in Nucleic Acid-Templated Reactions: Reaching Terminal Velocity

Authors
Published in Journal of the American Chemical Society. 2017, vol. 139, no. 4, p. 1444-1447
Abstract Nucleic acid-templated reactions have attracted significant attention for nucleic acid sensing and imaging. The level of signal amplification obtained from templated reactions is a function of the template turnover, wherein the template acts as the catalyst. Herein, we report the application of a pyridinium linker that immolates upon photocatalytic reduction with a ruthenium complex to yield the fastest nucleic acid templated reaction reported to date. We show that the templated reaction turnover is limited by the duplex dissociation kinetics beyond probes longer than a 6-mer and proceeded fastest for a 5-mer PNA probe. Using a beacon architecture that masks the catalytic template, we show that this methodology can be used for nucleic acid sensing extending the analyte recognition beyond a 5-mer. The system proceeds with a catalytic efficiency of 105 M–1 s–1 and achieves turnover frequency of >100 h–1.
Identifiers
Full text
Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Article (Author postprint) (799 Kb) - public document Free access
Structures
Research group Groupe Winssinger
Citation
(ISO format)
CHANG, Dalu, LINDBERG, Eric, WINSSINGER, Nicolas. Critical Analysis of Rate Constants and Turnover Frequency in Nucleic Acid-Templated Reactions: Reaching Terminal Velocity. In: Journal of the American Chemical Society, 2017, vol. 139, n° 4, p. 1444-1447. doi: 10.1021/jacs.6b12764 https://archive-ouverte.unige.ch/unige:91539

358 hits

3 downloads

Update

Deposited on : 2017-02-01

Export document
Format :
Citation style :