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Pro-survival role of gelsolin in mouse beta-cells

Tomas, Alejandra
Published in Diabetes. 2007, vol. 56, no. 1, p. 80-87
Abstract We have previously shown that the Ca(2+)-dependent actin-severing protein gelsolin plays an important role in regulated insulin secretion. The aim of this study was to determine the role of gelsolin in beta-cell survival as it has been shown to play a dual role in apoptosis in other cell types. MIN6 subclones B1 and C3, shown previously to express gelsolin at different levels (B1>C3 cells), were used for this purpose. We demonstrate that B1 cells have lower levels of apoptosis and active caspase-3 when compared with C3 cells, in both standard (25 mmol/l glucose and 15% FCS) and deprived (5 mmol/l glucose and 1% FCS) conditions. Overexpression of gelsolin resulted in a decrease in the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)(+) and active caspase-3(+) cells. Conversely, knockdown of gelsolin by RNA interference in B1 cells caused an increase in the number of TUNEL(+) and active caspase-3(+) cells. Finally, the anti-apoptotic role of gelsolin was confirmed in purified primary mouse beta-cells where overexpression of gelsolin resulted in a decrease in the percentage of TUNEL(+) cells. In summary, our results show for the first time that gelsolin plays a pro-survival role in pancreatic beta-cells.
Keywords AnimalsApoptosis/ physiologyCell Survival/ physiologyEnzyme-Linked Immunosorbent AssayGelsolin/genetics/ physiologyGene Expression RegulationInsulin-Secreting Cells/ cytology/ physiologyMiceRNA InterferenceRNA, Small Interfering/genetics
PMID: 17192468
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YERMEN, Barbara, TOMAS, Alejandra, HALBAN, Philippe A. Pro-survival role of gelsolin in mouse beta-cells. In: Diabetes, 2007, vol. 56, n° 1, p. 80-87. https://archive-ouverte.unige.ch/unige:9105

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Deposited on : 2010-07-12

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