en
Scientific article
English

Pro-survival role of gelsolin in mouse beta-cells

Published inDiabetes, vol. 56, no. 1, p. 80-87
Publication date2007
Abstract

We have previously shown that the Ca(2+)-dependent actin-severing protein gelsolin plays an important role in regulated insulin secretion. The aim of this study was to determine the role of gelsolin in beta-cell survival as it has been shown to play a dual role in apoptosis in other cell types. MIN6 subclones B1 and C3, shown previously to express gelsolin at different levels (B1>C3 cells), were used for this purpose. We demonstrate that B1 cells have lower levels of apoptosis and active caspase-3 when compared with C3 cells, in both standard (25 mmol/l glucose and 15% FCS) and deprived (5 mmol/l glucose and 1% FCS) conditions. Overexpression of gelsolin resulted in a decrease in the percentage of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)(+) and active caspase-3(+) cells. Conversely, knockdown of gelsolin by RNA interference in B1 cells caused an increase in the number of TUNEL(+) and active caspase-3(+) cells. Finally, the anti-apoptotic role of gelsolin was confirmed in purified primary mouse beta-cells where overexpression of gelsolin resulted in a decrease in the percentage of TUNEL(+) cells. In summary, our results show for the first time that gelsolin plays a pro-survival role in pancreatic beta-cells.

Keywords
  • Animals
  • Apoptosis/ physiology
  • Cell Survival/ physiology
  • Enzyme-Linked Immunosorbent Assay
  • Gelsolin/genetics/ physiology
  • Gene Expression Regulation
  • Insulin-Secreting Cells/ cytology/ physiology
  • Mice
  • RNA Interference
  • RNA, Small Interfering/genetics
Citation (ISO format)
YERMEN, Barbara, TOMAS, Alejandra, HALBAN, Philippe A. Pro-survival role of gelsolin in mouse beta-cells. In: Diabetes, 2007, vol. 56, n° 1, p. 80–87. doi: 10.2337/db06-0769
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0012-1797
435views
0downloads

Technical informations

Creation07/12/2010 12:02:27 PM
First validation07/12/2010 12:02:27 PM
Update time03/14/2023 3:53:23 PM
Status update03/14/2023 3:53:23 PM
Last indexation02/12/2024 6:58:49 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack