Protease-nexin I as an androgen-dependent secretory product of the murine seminal vesicle

Vassalli, Jean-Dominique; Huarte, Joachim; Bosco, Domenico; Sappino, Pascal; Sappino, N.; Velardi, A.; Wohlwend, Annelise Isabelle; Erno, H.; Monard, D.; Belin, Dominique

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Title		Protease-nexin I as an androgen-dependent secretory product of the murine seminal vesicle
Authors		Vassalli, Jean-Dominique Huarte, Joachim Bosco, Domenico Sappino, Pascal Sappino, N. Velardi, A. Wohlwend, Annelise Isabelle Erno, H. Monard, D. Belin, Dominique show all authors [1 - 10]
Published in		EMBO Journal. 1993, vol. 12, no. 5, p. 1871-1878
Abstract		A search for inhibitors of urokinase-type plasminogen activator (uPA) in the male and female murine genital tracts revealed high levels of a uPA ligand in the seminal vesicle. This ligand is functionally, biochemically and immunologically indistinguishable from protease-nexin I (PN-I), a serpin ligand of thrombin and uPA previously detected only in mesenchymal cells and astrocytes. A survey of murine tissues indicates that PN-I mRNA is most abundant in seminal vesicles, where it represents 0.2-0.4% of the mRNAs. PN-I is synthesized in the epithelium of the seminal vesicle, as determined by in situ hybridization, and is secreted in the lumen of the gland. PN-I levels are much lower in immature animals, and strongly decreased upon castration. Testosterone treatment of castrated males rapidly restores PN-I mRNA levels, indicating that PN-I gene expression is under androgen control.
Keywords		Amyloid beta-Protein Precursor — Animals — Base Sequence — Carrier Proteins/genetics/ secretion — Cells, Cultured — Dna — Female — Humans — Male — Mice — Molecular Sequence Data — Plasminogen Inactivators/genetics/ secretion — RNA, Messenger/genetics — Rats — Receptors, Cell Surface — Seminal Vesicles/ secretion — Sequence Homology, Nucleic Acid — Serpins/ secretion — Testosterone/physiology — Tumor Cells, Cultured — Urokinase-Type Plasminogen Activator/antagonists & inhibitors
Identifiers		PMID: 8491179
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC413407/pdf/emboj00077-0145.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		VASSALLI, Jean-Dominique et al. Protease-nexin I as an androgen-dependent secretory product of the murine seminal vesicle. In: EMBO Journal, 1993, vol. 12, n° 5, p. 1871-1878. https://archive-ouverte.unige.ch/unige:9070