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Pro-oxidant effects of nano-TiO2 on Chlamydomonas reinhardtii during short-term exposure

Koman, Volodymyr B.
Santschi, Christian
Martin, Olivier J. F.
Maurizi, Lionel
Jayaprakash, Amarnath
Bowen, Paul
Published in RSC Advances. 2016, vol. 6, no. 116, p. 115271-115283
Abstract This study sheds light on the short-term dynamics of pro-oxidant processes related to the exposure of C. reinhardtii microalgae to nano-TiO2 using a) conventional fluorescent probes for cellular pro-oxidant process and b) a recently developed cytochrome c biosensor for the continuous quantification of H2O2. The main aims are to investigate nano-TiO2 toxicity and the modifying factors thereof based on the paradigm of oxidative stress and to explore the utility of extracellular H2O2 as a potential biomarker of the observed cellular responses. This is the first study to provide continuous quantitative data on abiotic and biotic nano-TiO2-driven H2O2 generation to systematically investigate the link between extracellular and cellular pro-oxidant responses. Acute exposures of 1 h were performed in two different exposure media (MOPS and lake water), with particle concentrations from 10 mg L-1 to 200 mg L-1, with and without UV pre-illumination. Abiotic and biotic extracellular H2O2 were continuously measured with the biosensor and complemented with endpoints for abiotic ROS (H2DCF-DA), oxidative stress (CellROX® Green) and damage (propidium iodide) measured by flow cytometry at the beginning and end of exposure. Results showed that extracellular and intracellular pro-oxidant processes differed and that extracellular H2O2 cannot per se serve as a predictor of cellular oxidative stress or damage. The main predictors best describing the cellular responses included “exposure medium, “exposure time”, “UV treatment” as well as “exposure concentration”.
Keywords NanoecotoxicityMicroalgaeHydrogen peroxideOxidative stressOxidative damageBiosensingCytochrome cFlow cytometry
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Research groups Environmental Biogeochemistry and Ecotoxicology
ISE Pôle Sciences
Project FNS: 406440-131280
(ISO format)
VON MOOS, Nadia Rachel et al. Pro-oxidant effects of nano-TiO2 on Chlamydomonas reinhardtii during short-term exposure. In: RSC Advances, 2016, vol. 6, n° 116, p. 115271-115283. doi: 10.1039/C6RA16639C https://archive-ouverte.unige.ch/unige:90551

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Deposited on : 2016-12-20

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