Glucose-stimulated insulin secretion is coupled to the interaction of actin with the t-SNARE (target membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein) complex

Thurmond, D. C.; Gonelle-Gispert, Carmen; Furukawa, Megumi; Halban, Philippe A.; Pessin, J. E.

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Title		Glucose-stimulated insulin secretion is coupled to the interaction of actin with the t-SNARE (target membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein) complex
Authors		Thurmond, D. C. Gonelle-Gispert, Carmen Furukawa, Megumi Halban, Philippe A. Pessin, J. E.
Published in		Molecular Endocrinology. 2003, vol. 17, no. 4, p. 732-742
Abstract		The actin monomer sequestering agent latrunculin B depolymerized beta-cell cortical actin, which resulted in increased glucose-stimulated insulin secretion in both cultured MIN6 beta-cells and isolated rat islet cells. In perifused islets, latrunculin B treatment increased both first- and second-phase glucose-stimulated insulin secretion without any significant effect on total insulin content. This increase in secretion was independent of calcium regulation because latrunculin B also potentiated calcium-stimulated insulin secretion in permeabilized MIN6 cells. Confocal immunofluorescent microscopy revealed a redistribution of insulin granules to the cell periphery in response to glucose or latrunculin B, which correlated with a reduction in phalloidin staining of cortical actin. Moreover, the t-SNARE [target membrane soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor] proteins Syntaxin 1 and SNAP-25 coimmunoprecipitated polymerized actin from unstimulated MIN6 cells. Glucose stimulation transiently decreased the amount of actin coimmunoprecipitated with Syntaxin 1 and SNAP-25, and latrunculin B treatment fully ablated the coimmunoprecipitation. In contrast, the actin stabilizing agent jasplakinolide increased the amount of actin coimmunoprecipitated with the t-SNARE complex and prevented its dissociation upon glucose stimulation. These data suggest a mechanism whereby glucose modulates beta-cell cortical actin organization and disrupts the interaction of polymerized actin with the plasma membrane t-SNARE complex at a distal regulatory step in the exocytosis of insulin granules.
Keywords		Actins/drug effects/ metabolism — Animals — Antigens, Surface/metabolism — Bicyclo Compounds, Heterocyclic/pharmacology — Calcium/metabolism — Calcium Channels/metabolism — Cells, Cultured — Cytoplasmic Granules/metabolism — Glucose/ metabolism/pharmacology — Insulin/ secretion — Islets of Langerhans/drug effects/metabolism/secretion — Membrane Proteins/ metabolism — Nerve Tissue Proteins/metabolism — Perfusion — Precipitin Tests — R-SNARE Proteins — Rats — SNARE Proteins — Synaptosomal-Associated Protein 25 — Syntaxin 1 — Thiazoles/pharmacology — Thiazolidines — Vesicular Transport Proteins
Identifiers		DOI: 10.1210/me.2002-0333 PMID: 12554769
Full text		Article - Limited access to UNIGE Other version: http://mend.endojournals.org/cgi/reprint/17/4/732.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Citation (ISO format)		THURMOND, D. C. et al. Glucose-stimulated insulin secretion is coupled to the interaction of actin with the t-SNARE (target membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein) complex. In: Molecular Endocrinology, 2003, vol. 17, n° 4, p. 732-742. https://archive-ouverte.unige.ch/unige:9055