en
Scientific article
English

Replacement by a lacZ reporter gene assigns mouse connexin36, 45 and 43 to distinct cell types in pancreatic islets

Published inExperimental cell research, vol. 294, no. 1, p. 18-29
Publication date2004
Abstract

Transcripts of three connexin isoforms (Cx36, Cx43 and Cx45) have been reported in rodent pancreatic islets, but the precise distribution of the cognate proteins is still unknown. We determined expression of Cx36 in a cell-autonomous manner using mice with a targeted replacement of the Cx36 coding region by a lacZ reporter gene. For cell-autonomous monitoring of Cx43 expression, we used the Cre/loxP system: Mice carrying the Cx43 coding region flanked by loxP sites (floxed) also carried an embedded lacZ gene that is activated after Cre-mediated recombination in cells with transcriptional activity of the Cx43 gene. Deletion of the Cx43 coding region in beta-cells did not result in the activation of the embedded lacZ reporter gene. Instead, Cx43 expression was found in endothelial cells of the islets of Langerhans in mice with endothelium-specific deletion. Ubiquitous deletion of Cx43 led to a similar endothelial lacZ expression, but again, activity of the reporter gene was not detected in beta-cells. Mice with targeted replacement of the Cx45 coding region by lacZ showed a vascular expression similar to Cx43. The data show that native insulin-producing cells express a connexin isoform (Cx36) which differs from those (Cx43 and Cx45) expressed by vascular islet cells.

Keywords
  • Animals
  • Connexin 43/ analysis/genetics
  • Connexins/ analysis/genetics/metabolism
  • Endothelium, Vascular/chemistry
  • Gene Targeting
  • Genes, Reporter
  • Insulin/secretion
  • Islets of Langerhans/blood supply/ chemistry/cytology
  • Mice
  • Mice, Transgenic
  • Recombinant Fusion Proteins/analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Beta-Galactosidase/analysis/ genetics
Citation (ISO format)
THEIS, Martin et al. Replacement by a lacZ reporter gene assigns mouse connexin36, 45 and 43 to distinct cell types in pancreatic islets. In: Experimental cell research, 2004, vol. 294, n° 1, p. 18–29. doi: 10.1016/j.yexcr.2003.09.031
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0014-4827
601views
2downloads

Technical informations

Creation07/12/2010 12:01:06 PM
First validation07/12/2010 12:01:06 PM
Update time03/14/2023 3:53:02 PM
Status update03/14/2023 3:53:02 PM
Last indexation02/12/2024 6:57:41 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack