UNIGE document Scientific Article
previous document  unige:9034  next document
add to browser collection
Title

Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II

Authors
Sizonenko, S. V.
Published in Biochemical Journal. 1991, vol. 278 ( Pt 3), p. 621-625
Abstract Rat proinsulin I is converted into insulin more rapidly than is proinsulin II. To study this further, rat islets were labelled (10 min) and conversion kinetics of the labelled proinsulins were monitored during a 120 min chase. Proinsulins, conversion intermediates and both insulins were separated by h.p.l.c. The accumulation of des-64,65-(split proinsulin II) during the chase suggests that the B-chain/C-peptide junction of proinsulin II is cleaved more slowly than the equivalent site on proinsulin I. This accounts for the differential kinetics of conversion of proinsulins I and II, and is presumed to be caused by one (or more) of the amino acid replacements which distinguish the two proinsulins.
Keywords AnimalsC-Peptide/ metabolismCarboxypeptidase BCarboxypeptidases/metabolismChromatography, High Pressure LiquidInsulin/metabolismIslets of Langerhans/ metabolismKineticsMaleProinsulin/ metabolismRatsRats, Inbred StrainsTrypsin/metabolism
Identifiers
PMID: 1898351
Full text
Structures
Citation
(ISO format)
SIZONENKO, S. V., HALBAN, Philippe A. Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II. In: Biochemical Journal, 1991, vol. 278 ( Pt 3), p. 621-625. https://archive-ouverte.unige.ch/unige:9034

230 hits

0 download

Update

Deposited on : 2010-07-12

Export document
Format :
Citation style :