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Scientific article
English

Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II

Published inBiochemical journal, vol. 278 ( Pt 3), p. 621-625
Publication date1991
Abstract

Rat proinsulin I is converted into insulin more rapidly than is proinsulin II. To study this further, rat islets were labelled (10 min) and conversion kinetics of the labelled proinsulins were monitored during a 120 min chase. Proinsulins, conversion intermediates and both insulins were separated by h.p.l.c. The accumulation of des-64,65-(split proinsulin II) during the chase suggests that the B-chain/C-peptide junction of proinsulin II is cleaved more slowly than the equivalent site on proinsulin I. This accounts for the differential kinetics of conversion of proinsulins I and II, and is presumed to be caused by one (or more) of the amino acid replacements which distinguish the two proinsulins.

Keywords
  • Animals
  • C-Peptide/ metabolism
  • Carboxypeptidase B
  • Carboxypeptidases/metabolism
  • Chromatography, High Pressure Liquid
  • Insulin/metabolism
  • Islets of Langerhans/ metabolism
  • Kinetics
  • Male
  • Proinsulin/ metabolism
  • Rats
  • Rats, Inbred Strains
  • Trypsin/metabolism
Citation (ISO format)
SIZONENKO, S. V., HALBAN, Philippe A. Differential rates of conversion of rat proinsulins I and II. Evidence for slow cleavage at the B-chain/C-peptide junction of proinsulin II. In: Biochemical journal, 1991, vol. 278 ( Pt 3), p. 621–625. doi: 10.1042/bj2780621
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ISSN of the journal0264-6021
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