en
Scientific article
English

Role of the prohormone convertase PC2 in the processing of proglucagon to glucagon

Published inFEBS letters, vol. 413, no. 1, p. 119-123
Publication date1997
Abstract

Proglucagon is alternatively processed to glucagon in pancreatic alpha-cells, or to glucagon-like peptide-1 in intestinal L cells. Here, the specificity of PC2, the major prohormone convertase of alpha-cells, was examined both in vivo and in vitro. Adenovirus-mediated co-expression of proglucagon and PC2 in GH4C1 cells resulted in a pattern of processing products very similar to that observed in alpha-cells. Oxyntomodulin, an intermediate in the processing of proglucagon, was quantitatively converted to glucagon in vitro by purified recombinant PC2, in combination with carboxypeptidase E. It is concluded that PC2 is able to act alone in the pancreatic pathway of proglucagon processing.

Keywords
  • Animals
  • Carboxypeptidase H
  • Carboxypeptidases/pharmacology
  • Cells, Cultured
  • Glucagon/ metabolism
  • Glucagon-Like Peptides/metabolism
  • Immunoblotting
  • Oxyntomodulin
  • Pituitary Gland/ enzymology
  • Proglucagon
  • Proprotein Convertase 2
  • Protein Precursors/ metabolism
  • Rats
  • Subtilisins/ metabolism
  • Transfection
Citation (ISO format)
ROUILLÉ, Y. et al. Role of the prohormone convertase PC2 in the processing of proglucagon to glucagon. In: FEBS letters, 1997, vol. 413, n° 1, p. 119–123. doi: 10.1016/s0014-5793(97)00892-2
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ISSN of the journal0014-5793
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