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Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium

Marchetti, Anna
Brückert, Franz
Published in PloS one. 2016, vol. 11, no. 8, e0160376
Abstract Dictyostelium discoideum is a widely used model to study molecular mechanisms controlling cell adhesion, cell spreading on a surface, and phagocytosis. In this study we isolated and characterize a new mutant created by insertion of a mutagenic vector in the heretofore uncharacterized spdA gene. SpdA-ins mutant cells produce an altered, slightly shortened version of the SpdA protein. They spread more efficiently than WT cells when allowed to adhere to a glass substrate, and phagocytose particles more efficiently. On the contrary, a functional spdA knockout mutant where a large segment of the gene was deleted phagocytosed less efficiently and spread less efficiently on a substrate. These phenotypes were highly dependent on the cellular density, and were most visible at high cell densities, where secreted quorum-sensing factors inhibiting cell motility, spreading and phagocytosis are most active. These results identify the involvement of SpdA in the control of cell spreading and phagocytosis. The underlying molecular mechanisms, as well as the exact link between SpdA and cell spreading, remain to be established.
PMID: 27512991
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Research group Phagocytose (140)
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DIAS, Marco et al. Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium. In: PloS one, 2016, vol. 11, n° 8, p. e0160376. doi: 10.1371/journal.pone.0160376 https://archive-ouverte.unige.ch/unige:89993

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Deposited on : 2016-12-07

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