Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium

Article Article scientifique Article Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium eng 2016 author Dias Marco 583243 author Brochetta Cristiana 736516 author Marchetti Anna author Bodinier Romain 827889 author Brückert Franz author Cosson Pierre 0000-0002-4881-0358 119596 e0160376 10.1371/journal.pone.0160376 27512991 1932-6203 Dictyostelium discoideum is a widely used model to study molecular mechanisms controlling cell adhesion, cell spreading on a surface, and phagocytosis. In this study we isolated and characterize a new mutant created by insertion of a mutagenic vector in the heretofore uncharacterized spdA gene. SpdA-ins mutant cells produce an altered, slightly shortened version of the SpdA protein. They spread more efficiently than WT cells when allowed to adhere to a glass substrate, and phagocytose particles more efficiently. On the contrary, a functional spdA knockout mutant where a large segment of the gene was deleted phagocytosed less efficiently and spread less efficiently on a substrate. These phenotypes were highly dependent on the cellular density, and were most visible at high cell densities, where secreted quorum-sensing factors inhibiting cell motility, spreading and phagocytosis are most active. These results identify the involvement of SpdA in the control of cell spreading and phagocytosis. The underlying molecular mechanisms, as well as the exact link between SpdA and cell spreading, remain to be established. Dewey 612 134 Département de physiologie cellulaire et métabolisme 21C false 111 Phagocytose 140 PloS one 11 8 2b3738b8-1901-4ad0-a058-29b499056475 Article (Published version) Public journal.pone.0160376.PDF 1645532 application/pdf CC BY openAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Role of SpdA in Cell Spreading and Phagocytosis in Dictyostelium	PloS one			2016	560	229

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Brochetta, Cristiana