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Scientific article
English

The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells

Published inFEBS letters, vol. 581, no. 22, p. 4235-4240
Publication date2007
Abstract

The control of glucagon secretion by pancreatic alpha-cells is poorly understood, largely because of the difficulty to recognize living alpha-cells. We describe a new mouse model, referred to as GluCre-ROSA26EYFP (or GYY), allowing easy alpha-cell identification because of specific expression of EYFP. GYY mice displayed normal glycemic control during a fasting/refeeding test or intraperitoneal insulin injection. Glucagon secretion by isolated islets was normally inhibited by glucose and stimulated by adrenaline. [Ca(2+)](c) responses to arginine, adrenaline, diazoxide and tolbutamide, were similar in GYY and control mice. Hence, this new mouse model is a reliable and powerful tool to specifically study alpha-cells.

Keywords
  • Animals
  • Bacterial Proteins/ metabolism
  • Calcium/metabolism
  • Cell Survival
  • Glucagon/ metabolism/secretion
  • Glucagon-Secreting Cells/ cytology
  • Insulin/secretion
  • Insulin-Secreting Cells/cytology
  • Integrases/ metabolism
  • Luminescent Proteins/ metabolism
  • Mice
  • Mice, Transgenic
  • Models, Animal
  • Organ Specificity
Citation (ISO format)
QUOIX, Nicolas et al. The GluCre-ROSA26EYFP mouse: a new model for easy identification of living pancreatic alpha-cells. In: FEBS letters, 2007, vol. 581, n° 22, p. 4235–4240. doi: 10.1016/j.febslet.2007.07.068
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ISSN of the journal0014-5793
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