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Title

Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment

Authors
Amherdt, M.
Ravazzola, M.
Published in The Journal of Cell Biology. 1984, vol. 99, no. 6, p. 2187-2192
Abstract The secretion of insulin by the pancreatic B-cell involves a passage of the newly synthetized (pro)insulin polypeptides across the Golgi apparatus, at the trans pole of which secretory proteins are released as a population of secretory granules characterized by a clathrinlike coat on segments of their limiting membrane. When the conversion of radiolabeled proinsulin to insulin was inhibited by replacing arginine and lysine with the aminoacid analogs, canavanine and thialysine, the nonconverted radioactive material remained associated with Golgi-derived, coated secretory granules. The coat was characterized as clathrin-containing by immunocytochemistry. Under analog treatment, the noncoated, storage secretory granules did not become markedly labeled during the pulse-chase experiment. These data are compatible with the hypothesis that in normal conditions, the maturation of the coated compartment into noncoated granules is linked to the effective conversion of the prohormone.
Keywords AnimalsAutoradiographyClathrin/ physiologyCysteine/ analogs & derivatives/diagnostic useGolgi Apparatus/ metabolism/ultrastructureIntracellular Membranes/ metabolism/ultrastructureIslets of Langerhans/ metabolism/ultrastructureKineticsMicroscopy, ElectronProinsulin/ metabolismRatsTritium/diagnostic use
Identifiers
PMID: 6389572
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Other version: http://jcb.rupress.org/content/99/6/2187.full.pdf
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ORCI, Lelio et al. Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment. In: The Journal of Cell Biology, 1984, vol. 99, n° 6, p. 2187-2192. https://archive-ouverte.unige.ch/unige:8945

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Deposited on : 2010-07-12

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