en
Scientific article
English

Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment

Published inThe Journal of cell biology, vol. 99, no. 6, p. 2187-2192
Publication date1984
Abstract

The secretion of insulin by the pancreatic B-cell involves a passage of the newly synthetized (pro)insulin polypeptides across the Golgi apparatus, at the trans pole of which secretory proteins are released as a population of secretory granules characterized by a clathrinlike coat on segments of their limiting membrane. When the conversion of radiolabeled proinsulin to insulin was inhibited by replacing arginine and lysine with the aminoacid analogs, canavanine and thialysine, the nonconverted radioactive material remained associated with Golgi-derived, coated secretory granules. The coat was characterized as clathrin-containing by immunocytochemistry. Under analog treatment, the noncoated, storage secretory granules did not become markedly labeled during the pulse-chase experiment. These data are compatible with the hypothesis that in normal conditions, the maturation of the coated compartment into noncoated granules is linked to the effective conversion of the prohormone.

Keywords
  • Animals
  • Autoradiography
  • Clathrin/ physiology
  • Cysteine/ analogs & derivatives/diagnostic use
  • Golgi Apparatus/ metabolism/ultrastructure
  • Intracellular Membranes/ metabolism/ultrastructure
  • Islets of Langerhans/ metabolism/ultrastructure
  • Kinetics
  • Microscopy, Electron
  • Proinsulin/ metabolism
  • Rats
  • Tritium/diagnostic use
Citation (ISO format)
ORCI, Lelio et al. Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment. In: The Journal of cell biology, 1984, vol. 99, n° 6, p. 2187–2192. doi: 10.1083/jcb.99.6.2187
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0021-9525
520views
0downloads

Technical informations

Creation07/12/2010 11:59:23 AM
First validation07/12/2010 11:59:23 AM
Update time03/14/2023 3:52:19 PM
Status update03/14/2023 3:52:19 PM
Last indexation02/12/2024 6:55:25 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack