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Title

Slow cleavage at the proinsulin B-chain/connecting peptide junction associated with low levels of endoprotease PC1/3 in transformed beta cells

Authors
Sizonenko, S. V.
Published in Journal of Biological Chemistry. 1993, vol. 268, no. 22, p. 16098-16100
Abstract Conversion of rat proinsulins I and II was slower in transformed INS cells than in primary (islet) beta cells, with accumulation of des-64,65 but no detectable des-31,32-split proinsulin, indicating slow cleavage at the B-chain/connecting peptide (C-peptide) junction. Western blot analysis showed lower levels of the endoprotease PC1/3 in INS cells than in beta cells, as well as a 4-fold reduction in the ratio of PC1/3 to PC2, thus supporting the hypothesis that PC1/3 is the endoprotease responsible for cleavage at the B-chain/C-peptide junction.
Keywords AnimalsAspartic Acid Endopeptidases/ metabolismC-Peptide/genetics/ metabolismCell LineIslets of Langerhans/cytology/metabolismKineticsMaleProprotein ConvertasesRatsRats, Sprague-Dawley
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PMID: 8393853
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Other version: http://www.jbc.org/content/268/22/16098.full.pdf
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NEERMAN ARBEZ, Marguerite, SIZONENKO, S. V., HALBAN, Philippe A. Slow cleavage at the proinsulin B-chain/connecting peptide junction associated with low levels of endoprotease PC1/3 in transformed beta cells. In: Journal of Biological Chemistry, 1993, vol. 268, n° 22, p. 16098-16100. https://archive-ouverte.unige.ch/unige:8936

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Deposited on : 2010-07-12

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