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Scientific article
English

Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model

Contributors

Chavez Galan, Leslie; Vesin, Marie-Dominique; Segueni, Noria; Prasad, Pritha; Buser Llinares, Raphaële; Blaser, Guillaume; Pache, Jean-Claude; Ryffel, Bernhard; Quesniaux, Valérie F J; Garcia, Irène

Published in

The American journal of pathology, vol. 186, no. 9, p. 2364-2377

Publication date

2016

Abstract

Tumor necrosis factor (TNF) is crucial to control Mycobacterium tuberculosis infection, which remains a leading cause of morbidity and mortality worldwide. TNF blockade compromises host immunity and may cause reactivation of latent infection, resulting in overt pulmonary, pleural, and extrapulmonary tuberculosis. Herein, we investigate the roles of TNF and TNF receptors in the control of Mycobacterium bovis bacillus Calmette-Guerin (BCG) pleural infection in a murine model. As controls, wild-type mice and those with a defective CCR5, a receptor that is crucial for control of viral infection but not for tuberculosis, were used. BCG-induced pleural infection was uncontrolled and progressive in absence of TNF or TNF receptor 1 (TNFR1)/TNFR2 (TNFR1R2) with increased inflammatory cell recruitment and bacterial load in the pleural cavity, and heightened levels of pleural and serum proinflammatory cytokines and chemokines, compared to wild-type control mice. The visceral pleura was thickened with chronic inflammation, which was prominent in TNF(-/-) and TNFR1R2(-/-) mice. The parietal pleural of TNF(-/-) and TNFR1R2(-/-) mice exhibited abundant inflammatory nodules containing mycobacteria, and these mice developed nonresolving inflammation and succumbed from disseminated BCG infection. By contrast, CCR5(-/-) mice survived and controlled pleural BCG infection as wild-type control mice. In conclusion, BCG-induced pleurisy was uncontrolled in the absence of TNF or TNF receptors with exacerbated inflammatory response, impaired bacterial clearance, and defective mesothelium repair, suggesting a critical role of TNF to control mycobacterial pleurisy.

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

CHAVEZ GALAN, Leslie et al. Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model. In: The American journal of pathology, 2016, vol. 186, n° 9, p. 2364–2377. doi: 10.1016/j.ajpath.2016.05.015

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Article (Published version)

Identifiers

Journal ISSN

0002-9440
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Technical informations

Creation

17/10/2016 16:11:00

First validation

17/10/2016 16:11:00

Update

15/03/2023 00:57:17

Status update

15/03/2023 00:57:17

Last indexation

31/10/2024 05:06:11
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