UNIGE document Scientific Article
previous document  unige:89090  next document
add to browser collection
Title

Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model

Authors
Segueni, Noria
Ryffel, Bernhard
show hidden authors show all authors [1 - 10]
Published in The American Journal of Pathology. 2016, vol. 186, no. 9, p. 2364-2377
Abstract Tumor necrosis factor (TNF) is crucial to control Mycobacterium tuberculosis infection, which remains a leading cause of morbidity and mortality worldwide. TNF blockade compromises host immunity and may cause reactivation of latent infection, resulting in overt pulmonary, pleural, and extrapulmonary tuberculosis. Herein, we investigate the roles of TNF and TNF receptors in the control of Mycobacterium bovis bacillus Calmette-Guerin (BCG) pleural infection in a murine model. As controls, wild-type mice and those with a defective CCR5, a receptor that is crucial for control of viral infection but not for tuberculosis, were used. BCG-induced pleural infection was uncontrolled and progressive in absence of TNF or TNF receptor 1 (TNFR1)/TNFR2 (TNFR1R2) with increased inflammatory cell recruitment and bacterial load in the pleural cavity, and heightened levels of pleural and serum proinflammatory cytokines and chemokines, compared to wild-type control mice. The visceral pleura was thickened with chronic inflammation, which was prominent in TNF(-/-) and TNFR1R2(-/-) mice. The parietal pleural of TNF(-/-) and TNFR1R2(-/-) mice exhibited abundant inflammatory nodules containing mycobacteria, and these mice developed nonresolving inflammation and succumbed from disseminated BCG infection. By contrast, CCR5(-/-) mice survived and controlled pleural BCG infection as wild-type control mice. In conclusion, BCG-induced pleurisy was uncontrolled in the absence of TNF or TNF receptors with exacerbated inflammatory response, impaired bacterial clearance, and defective mesothelium repair, suggesting a critical role of TNF to control mycobacterial pleurisy.
Identifiers
PMID: 27456129
Full text
Article (Published version) (6.7 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Pache Jean-Claude (pathologie clinique) (658)
Citation
(ISO format)
CHAVEZ GALAN, Leslie et al. Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model. In: The American Journal of Pathology, 2016, vol. 186, n° 9, p. 2364-2377. https://archive-ouverte.unige.ch/unige:89090

61 hits

0 download

Update

Deposited on : 2016-11-18

Export document
Format :
Citation style :