Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model

Article Article scientifique Article Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model eng 2016 author Chavez Galan Leslie 814837 author Vesin Marie-Dominique 65117 author Segueni Noria author Prasad Pritha 805233 author Buser Llinares Raphaële 83624 author Blaser Guillaume 583057 author Pache Jean-Claude 513830 author Ryffel Bernhard author Quesniaux Valérie F J author Garcia Irène 26048 2364-2377 10.1016/j.ajpath.2016.05.015 27456129 0002-9440 Tumor necrosis factor (TNF) is crucial to control Mycobacterium tuberculosis infection, which remains a leading cause of morbidity and mortality worldwide. TNF blockade compromises host immunity and may cause reactivation of latent infection, resulting in overt pulmonary, pleural, and extrapulmonary tuberculosis. Herein, we investigate the roles of TNF and TNF receptors in the control of Mycobacterium bovis bacillus Calmette-Guerin (BCG) pleural infection in a murine model. As controls, wild-type mice and those with a defective CCR5, a receptor that is crucial for control of viral infection but not for tuberculosis, were used. BCG-induced pleural infection was uncontrolled and progressive in absence of TNF or TNF receptor 1 (TNFR1)/TNFR2 (TNFR1R2) with increased inflammatory cell recruitment and bacterial load in the pleural cavity, and heightened levels of pleural and serum proinflammatory cytokines and chemokines, compared to wild-type control mice. The visceral pleura was thickened with chronic inflammation, which was prominent in TNF(-/-) and TNFR1R2(-/-) mice. The parietal pleural of TNF(-/-) and TNFR1R2(-/-) mice exhibited abundant inflammatory nodules containing mycobacteria, and these mice developed nonresolving inflammation and succumbed from disseminated BCG infection. By contrast, CCR5(-/-) mice survived and controlled pleural BCG infection as wild-type control mice. In conclusion, BCG-induced pleurisy was uncontrolled in the absence of TNF or TNF receptors with exacerbated inflammatory response, impaired bacterial clearance, and defective mesothelium repair, suggesting a critical role of TNF to control mycobacterial pleurisy. Dewey 616.07 137 Département de pathologie et immunologie 21F false 392 Groupe Pache Jean-Claude (pathologie clinique) 658 254 Rôles des cytokines de la famille du TNF dans les infections mycobactériennes 500 The American journal of pathology 186 9 d0cbc84f-0abc-438e-9632-67d2cd432c52 Article (Published version) Restricted Tumor necrosis factor and its receptors are crucial to control mycobacterium bovis bacillus Calmette-Guerin pleural infection in a murine model.pdf 7068362 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Tumor Necrosis Factor and Its Receptors Are Crucial to Control Mycobacterium bovis Bacillus Calmette-Guerin Pleural Infection in a Murine Model	The American journal of pathology			2016	528	1

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