

Other version: http://www.jbc.org/content/282/46/33530.full.pdf
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Dynamin is functionally coupled to insulin granule exocytosis |
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Published in | The Journal of biological chemistry. 2007, vol. 282, no. 46, p. 33530-33536 | |
Abstract | The insulin granule integral membrane protein marker phogrin-green fluorescent protein was co-localized with insulin in Min6B1 beta-cell secretory granules but did not undergo plasma membrane translocation following glucose stimulation. Surprisingly, although expression of a dominant-interfering dynamin mutant (Dyn/K44A) inhibited transferrin receptor endocytosis, it had no effect on phogringreen fluorescent protein localization in the basal or secretagogue-stimulated state. By contrast, co-expression of Dyn/K44A with human growth hormone as an insulin secretory marker resulted in a marked inhibition of human growth hormone release by glucose, KCl, and a combination of multiple secretagogues. Moreover, serial pulse depolarization stimulated an increase in cell surface capacitance that was also blocked in cells expressing Dyn/K44A. Similarly, small interference RNA-mediated knockdown of dynamin resulted in marked inhibition of glucose-stimulated insulin secretion. Together, these data suggest the presence of a selective kiss and run mechanism of insulin release. Moreover, these data indicate a coupling between endocytosis and exocytosis in the regulation of beta-cell insulin secretion. | |
Keywords | Animals — Cell Line — Cell Membrane/metabolism — Dynamins/genetics/ physiology — Exocytosis — Green Fluorescent Proteins/metabolism — Insulin/ metabolism — Insulin-Secreting Cells/metabolism — Mice — Models, Biological — Mutation — Potassium Chloride/chemistry — Protein Transport — RNA, Small Interfering/metabolism | |
Identifiers | PMID: 17848579 | |
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Citation (ISO format) | MIN, Le et al. Dynamin is functionally coupled to insulin granule exocytosis. In: The Journal of biological chemistry, 2007, vol. 282, n° 46, p. 33530-33536. doi: 10.1074/jbc.M703402200 https://archive-ouverte.unige.ch/unige:8902 |