Dynamin is functionally coupled to insulin granule exocytosis

Min, Le; Leung, Y. M.; Tomas, Alejandra; Watson, R. T.; Gaisano, H. Y.; Halban, Philippe A.; Pessin, J. E.; Hou, J. C.

doi:10.1074/jbc.M703402200

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Title		Dynamin is functionally coupled to insulin granule exocytosis
Authors		Min, Le Leung, Y. M. Tomas, Alejandra Watson, R. T. Gaisano, H. Y. Halban, Philippe A. Pessin, J. E. Hou, J. C.
Published in		Journal of Biological Chemistry. 2007, vol. 282, no. 46, p. 33530-33536
Abstract		The insulin granule integral membrane protein marker phogrin-green fluorescent protein was co-localized with insulin in Min6B1 beta-cell secretory granules but did not undergo plasma membrane translocation following glucose stimulation. Surprisingly, although expression of a dominant-interfering dynamin mutant (Dyn/K44A) inhibited transferrin receptor endocytosis, it had no effect on phogringreen fluorescent protein localization in the basal or secretagogue-stimulated state. By contrast, co-expression of Dyn/K44A with human growth hormone as an insulin secretory marker resulted in a marked inhibition of human growth hormone release by glucose, KCl, and a combination of multiple secretagogues. Moreover, serial pulse depolarization stimulated an increase in cell surface capacitance that was also blocked in cells expressing Dyn/K44A. Similarly, small interference RNA-mediated knockdown of dynamin resulted in marked inhibition of glucose-stimulated insulin secretion. Together, these data suggest the presence of a selective kiss and run mechanism of insulin release. Moreover, these data indicate a coupling between endocytosis and exocytosis in the regulation of beta-cell insulin secretion.
Keywords		Animals — Cell Line — Cell Membrane/metabolism — Dynamins/genetics/ physiology — Exocytosis — Green Fluorescent Proteins/metabolism — Insulin/ metabolism — Insulin-Secreting Cells/metabolism — Mice — Models, Biological — Mutation — Potassium Chloride/chemistry — Protein Transport — RNA, Small Interfering/metabolism
Identifiers		DOI: 10.1074/jbc.M703402200 PMID: 17848579
Full text		Article - Limited access to UNIGE Other version: http://www.jbc.org/content/282/46/33530.full.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		MIN, Le et al. Dynamin is functionally coupled to insulin granule exocytosis. In: Journal of Biological Chemistry, 2007, vol. 282, n° 46, p. 33530-33536. doi: 10.1074/jbc.M703402200 https://archive-ouverte.unige.ch/unige:8902